FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740441
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACTCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC26420.3568143849408663No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25790.3483059419994301No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23030.3110308586369474No Hit
GACTTAGGACGGTCAATTTGGTCCCTCCGCCGAATACTTGAGCACCATCATAGTAGACCAGGCAGTAATAGTCAG22500.3038729621941519No Hit
GTCCAGGAGTGTCTCTTGTTTGTACTATAAATCAGTGCCCTGGGTGGTTG20600.27821257871997906No Hit
ACCCTGTACTGGTCATCTATAACGATAGCAACCGGCCCTCAGGGATCCCT19590.26457205908370823No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18760.25336252314499064No Hit
GTACAGGGTCCTGGCCTGGCTTTTGATGGCACCAGTGCACATCCTTACTTCCAATGTTGTTTCCCCCACAGGTGA16960.22905268616945848No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16490.2227051176258473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGT16270.2197339153288378No Hit
CCATCATAGTAGACCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTG15900.21473689328386733No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15710.21217085493645005No Hit
GACTATTACTGCCTGGTCTACTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAG15400.20798416079066392No Hit
CTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGT15380.2077140514909358No Hit
GATTTATAGTACAAACAAGAGACACTCCTGGACTCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC15130.2043376852443341No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAATTTGGTCCCTC14860.2006912096980043No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14820.20015099109854806No Hit
CTATTACTGTCCCACACCCGACAGTAATAGTCAGCCTCATCCCCAGCCTC14480.19555913300316974No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13850.1870506900617335No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13750.1857001435630928No Hit
GCCTTGGGCTGACTTAGGACGGTCAATTTGGTCCCTCCGCCGAATACTTG13500.1823237773164911No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13400.18097323081785044No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC13130.17732675527152064No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG12740.17205962392682198No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC12730.17192456927695793No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12540.16935853092954065No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12300.16611721933280302No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGTGATCACTA12300.16611721933280302No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12270.16571205538321082No Hit
CCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCACAGATG12100.16341612633552166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11950.16139030658756065No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11740.15855415894041525No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11560.156123175242862No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGTGATCACTATTACTGTCCCACACC11520.15558295664340577No Hit
GGCCAGGACCCTGTACTGGTCATCTATAACGATAGCAACCGGCCCTCAGG11500.15531284734367762No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11110.15004571599897898No Hit
GGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11080.14964055204938678No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10810.14599407650305696No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10700.14450847535455222No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10620.14342803815563968No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10320.13937639865971765No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10130.13681036031230037No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAATAATTA9850.13302883011610647No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9800.13235355686678615No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9710.13113806501800954No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9440.1274915894716797No Hit
GTCTACTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC9390.1268163162223594No Hit
CTATAAATCAGTGCCCTGGGTGGTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTAATTATTGGTGACTGCT9380.12668126157249532No Hit
GTAATAGTGATCACCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA9380.12668126157249532No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACC9230.12465544182453431No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9100.12289973137630142No Hit
CACTATTACTGTCCCACACCCGACAGTAATAGTCAGCCTCATCCCCAGCCTCGATCCTCCTGATGGTTAGGGTGG9030.12195434882725294No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9010.12168423952752482No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8990.12141413022779668No Hit
CAGTAATAGTGATCACCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC8840.11938831047983565No Hit
CTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8830.1192532558299716No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8770.11844292793078719No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8660.11695732678228246No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8610.11628205353296212No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8560.11560678028364178No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC8540.11533667098391363No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCATAGAGGTCAGCTTCAGGGAGA8450.11412117913513703No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8430.1138510698354089No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG8410.11358096053568076No Hit
GCACTGGAGCAGTCACCAATAATTACTATCCAAACTGGTTCCAGCAGAAA8400.1134459058858167No Hit
CTGCACAGGCTCTGCGACCTCCTATGAGCTGACTCAGCCACACTCAGTGT8380.11317579658608856No Hit
ATAGTAGACCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTG8230.11114997683812755No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8050.10871899314057433No Hit
GTCTCTGGTGGCTCCGTCAGCACTGATGGTTACTACTGGACCTGGGTCCG8050.10871899314057433No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8020.10831382919098213No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGGTCTACTATGATGGTGCTC8000.108043719891254No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7760.10480240829451638No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGAGTC7750.10466735364465232No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC7670.10358691644573977No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7570.1022363699470991No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAATTTGGTCCCTCCGCCGAATACTTGAG7540.10183120599750689No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7500.10129098739805062No Hit
GACTATTACTGTCGGGTGTGGGACAGTAATAGTGATCACCGGGTGTTCGG7500.10129098739805062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7490.10115593274818656No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTAT7450.10061571414873029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG150.002092454371.0889365
GTGGTAT1100.056.1408231
TATCTCA200.007080022252.2564350
AACGAAT200.0074080851.6612835
AATGGTA4300.047.467570
TGGTATC1350.045.7443732
GTATCAA12200.043.3071751
GGTATCA4050.042.35591
CGGGTCG350.00138446939.21211218
CCAATAG350.001384928639.2094651
ACGGGAC557.675306E-637.4297416
TATCAAC14200.036.7243542
CAACGCA14650.036.0671125
ATCAACG14650.035.5963023
TCAACGC14700.035.4752274
AACGCAG14900.035.4619566
CCTAGTA400.00236509235.16070659
ACGGGTT400.002666763334.3105965
ACGGGTA905.47152E-934.31059616
ACGCAGA15800.033.4419757
Produced by FastQC (version 0.11.5)