FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740441
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27210.36748370228012767No Hit
CTCCTGGACTCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC25770.34803583269970195No Hit
GACTTAGGACGGTCAATTTGGTCCCTCCGCCGAATACTTGAGCACCATCATAGTAGACCAGGCAGTAATAGTCAG21230.28672102166141533No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20110.27159490087663973No Hit
ACCCTGTACTGGTCATCTATAACGATAGCAACCGGCCCTCAGGGATCCCT18860.2547130696436313No Hit
GTCCAGGAGTGTCTCTTGTTTGTACTATAAATCAGTGCCCTGGGTGGTTG18360.24796033715042792No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17690.2389116756095354No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16490.2227051176258473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGT15890.21460183863400323No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15810.2135214014350907No Hit
GTACAGGGTCCTGGCCTGGCTTTTGATGGCACCAGTGCACATCCTTACTTCCAATGTTGTTTCCCCCACAGGTGA15330.20703877824161548No Hit
CCATCATAGTAGACCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTG15080.20366241199501378No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14920.2015015375971887No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA14660.19799011670072292No Hit
GACTATTACTGCCTGGTCTACTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAG14430.1948838597538494No Hit
GATTTATAGTACAAACAAGAGACACTCCTGGACTCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC14410.19461375045412124No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC14050.18975178305901483No Hit
CTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGT14010.18921156445955856No Hit
GCCTTGGGCTGACTTAGGACGGTCAATTTGGTCCCTCCGCCGAATACTTG13800.18637541681241315No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13520.18259388661621925No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC13230.17867730177016128No Hit
CTATTACTGTCCCACACCCGACAGTAATAGTCAGCCTCATCCCCAGCCTC12730.17192456927695793No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAATTTGGTCCCTC12190.16463161818429828No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12010.16220063448674507No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11910.16085008798810438No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11870.1603098693886481No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11630.1570685577919105No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG11500.15531284734367762No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGTGATCACTATTACTGTCCCACACC11270.15220659039680406No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11200.1512612078477556No Hit
CCTATGAGCTGACTCAGCCACACTCAGTGTCAGTGGCCACAGCACAGATG10880.14693945905210543No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGTGATCACTA10850.14653429510251323No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10840.14639924045264915No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10760.14531880325373664No Hit
GGTATGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10500.14180738235727086No Hit
GGCCAGGACCCTGTACTGGTCATCTATAACGATAGCAACCGGCCCTCAGG10440.14099705445808647No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10030.1354598138136597No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9940.1342443219648831No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9900.13370410336542682No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9840.13289377546624243No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTATGTCTCATAAGTGACTTCTACC9810.1324886115166502No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9800.13235355686678615No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9780.132083447567058No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9320.1258709336733109No Hit
GTCTACTATGATGGTGCTCAAGTATTCGGCGGAGGGACCAAATTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC9310.12573587902344685No Hit
GTAATAGTGATCACCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA9310.12573587902344685No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9190.12411522322507804No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG9090.12276467672643736No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9090.12276467672643736No Hit
CACTATTACTGTCCCACACCCGACAGTAATAGTCAGCCTCATCCCCAGCCTCGATCCTCCTGATGGTTAGGGTGG8880.11992852907929193No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8840.11938831047983565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8840.11938831047983565No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAATAATTA8830.1192532558299716No Hit
GTCTCTGGTGGCTCCGTCAGCACTGATGGTTACTACTGGACCTGGGTCCG8600.11614699888309804No Hit
CTGCACAGGCTCTGCGACCTCCTATGAGCTGACTCAGCCACACTCAGTGT8580.1158768895833699No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGGTCTACTATGATGGTGCTC8560.11560678028364178No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8490.1146613977345933No Hit
CTATAAATCAGTGCCCTGGGTGGTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTAATTATTGGTGACTGCT8430.1138510698354089No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC8430.1138510698354089No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8320.11236546868690417No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8290.11196030473731196No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8210.11087986753839942No Hit
CTTATGAGACATACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8200.11074481288853534No Hit
CAGTAATAGTGATCACCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC8160.11020459428907907No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA8040.10858393849071027No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCATAGAGGTCAGCTTCAGGGAGA7910.1068282280424774No Hit
GCACTGGAGCAGTCACCAATAATTACTATCCAAACTGGTTCCAGCAGAAA7910.1068282280424774No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTAT7790.1052075722441086No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7760.10480240829451638No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7570.1022363699470991No Hit
ATAGTAGACCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTG7570.1022363699470991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1250.054.8229681
TGGTATC1500.045.679632
GGTATCA4450.043.1191861
GTATCAA12150.039.481561
TATCAAC13000.036.895092
AATGGTA4350.035.03619470
ACGGGCT601.4005409E-534.2851916
GATTGAC400.002684456334.2643551
ATCAACG14000.034.2597243
CAACGCA14050.034.1447225
AACGCAG14100.034.0351376
TCAACGC14100.034.016754
ACGCAGA14600.032.871777
GCCCACG652.1210803E-532.26717853
CAGAGTA15050.031.90182710
AGAGTAC15100.031.56907811
GCAGAGT15400.031.1746799
TTACACC1001.5505066E-830.8337524
GGGACGT450.004755564530.48808920
ACGGGAT1401.6370905E-1129.3873116
Produced by FastQC (version 0.11.5)