FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580697
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT51560.8878985081720759No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA37420.6443980251318674No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTCCTGCACCGGCAGCGGTGGCAGCATTGCCAC36820.6340656142532164No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA31150.5364243314499645No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29570.5092156494695168No Hit
GACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGT28150.4847622770567095No Hit
GTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTCCTGCACCGGCAGCGGTGGCAGCATTGCCACCAACTATGTG26950.46409745529940744No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26030.4482544252854759No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25550.4399884965825551No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24850.42793401722412894No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24430.42070132960907325No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23430.4034806448113216No Hit
ACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGC22520.3878098216453676No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21970.37833844500660413No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGA20150.3469967986746961No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19660.3385586631237978No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19600.3375254220359327No Hit
GACTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGT18850.32460990843761894No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAAC17680.3044617072242495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16640.28655219503458773No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16090.2770808183958243No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG15900.27380888828425154No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15870.27329226774031895No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15420.26554295958133073No Hit
CTCCTGCACCGGCAGCGGTGGCAGCATTGCCACCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC14920.25693261718245486No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTC14880.2562437897905448No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14220.24487813782402873No Hit
GTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA13990.24091738032054585No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACA13880.23902310499279317No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13180.22696862563436698No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12850.22128579965110892No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT12310.21198662986032304No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11920.2052705627891999No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11390.1961435998463915No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11360.19562697930245893No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA11320.19493815191054886No Hit
CTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCC11260.1939049108226838No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA11230.19338829027875123No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11150.19201063549493108No Hit
GACATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGT10940.18839429168740324No Hit
CTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC10880.18736105059953814No Hit
CTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTG10870.1871888437515606No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGGGGTCC10720.1846057410318979No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAG10610.1827114657041452No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA10560.1818504314642576No Hit
GGAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCAT10540.18150601776830258No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT10520.18116160407234755No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10430.1796117424405499No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10330.17788967396077474No Hit
CCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGA10160.17496215754515695No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAGG9950.17134581373762908No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCT9810.16893491786594386No Hit
CTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCT9680.16669622884223614No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9650.16617960829830358No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9640.16600740145032605No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC9590.1651463672104385No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9410.1620466439468432No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT9410.1620466439468432No Hit
GTCCTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC9380.16153002340291064No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9340.16084119601100058No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG9240.15911912753122542No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9220.15877471383527036No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT9090.15653602481156265No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9040.15567499057167508No Hit
CATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC9000.154986163179765No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8940.1539529220918999No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGAT8890.15309188785201233No Hit
CACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTC8820.1518864399161697No Hit
ACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCA8780.15119761252425964No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8680.1494755440444845No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT8530.14689244132482174No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC8430.14517037284504655No Hit
GCCACCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCAC8430.14517037284504655No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8310.14310389066931636No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG8300.14293168382133883No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8290.1427594769733613No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8220.1415540290375187No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAG8220.1415540290375187No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7930.1365600304461707No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG7870.13552678935830562No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7850.13518237566235058No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7730.13311589348662037No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA7660.13191044555077777No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7600.13087720446291268No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7590.13070499761493515No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7510.12932734283111502No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG7440.1281218948952724No Hit
GGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7410.12760527435133986No Hit
GGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCAC7300.12571099902358718No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7260.12502217163167711No Hit
GTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATA7250.1248499647836996No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA7190.12381672369583449No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC7100.12226686206403684No Hit
CCCCAGAGGGTCTTTCCTTATCCTCAAAGATGACATTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCA7070.12175024152010429No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA7050.12140582782414926No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7030.12106141412819421No Hit
GACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATCT6980.12020037988830663No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC6940.11951155249639658No Hit
GTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTT6930.11933934564841905No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTC6920.11916713880044154No Hit
CAGGAAGCAGAGCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTT6860.11813389771257643No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA6820.11744507032066638No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGA6730.11589520868886873No Hit
CCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGA6680.11503417444898115No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC6620.11400093336111604No Hit
GTATATTACTGTGCGAGAGATCCGGCGCGGGGAATGTGGAACTGGAACTA6590.11348431281718349No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6570.11313989912122846No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT6560.11296769227325093No Hit
CTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG6560.11296769227325093No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6520.11227886488134088No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC6510.11210665803336335No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG6450.11107341694549826No Hit
CTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCT6440.11090121009752073No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG6380.10986796900965565No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT6270.10797369368190295No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG6230.1072848662899929No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC6210.10694045259403787No Hit
GGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGG6180.1064238320501053No Hit
ACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGC6170.10625162520212779No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG6110.10521838411426268No Hit
CAGTAACCATCTCCTGCACCGGCAGCGGTGGCAGCATTGCCACCAACTAT6110.10521838411426268No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG6030.10384072933044255No Hit
CCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCC5950.10246307454662243No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAG5930.1021186608506674No Hit
GTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGGGGTC5850.10074100606684724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAG306.6776614E-445.4726427
ACAGGTA306.754695E-445.3669171
GGTATCA4950.044.679541
GTGGTAT1250.040.8302271
CAGAGGG15700.039.1055270
GCGCTCA350.001402986439.10480533
TAGCGCT350.001413769239.04394531
CTAGCGC350.001416174339.0304530
TCTTATA350.001442234638.885932
GTATCAA11500.035.208671
ACTAGCG400.002733468834.1368929
GACTAGC400.00274506834.10742628
CGAGACT400.002759038534.07213225
ATCTCCG400.002777753234.0251912
GCTTGAC400.002777753234.025191
TACTGAC400.002777753234.025197
TGGTATC1500.034.025192
TCAATGA1650.032.994122
AATATAC1800.032.1349033
ATACGGC1800.032.1349036
Produced by FastQC (version 0.11.5)