FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580697
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGGACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT48600.836925281170731No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA38120.6564525044902936No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTCCTGCACCGGCAGCGGTGGCAGCATTGCCAC31530.5429681916731101No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30040.5173093713244601No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27470.4730522113942383No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26610.4582424224681718No Hit
GACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGT25950.44687677050165575No Hit
GTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTCCTGCACCGGCAGCGGTGGCAGCATTGCCACCAACTATGTG25790.44412146093401556No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24760.4263841555923313No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23700.40813022970671453No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22330.38453789153379475No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22270.38350465044592963No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21390.3683504478239082No Hit
ACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGC20210.3480300397625612No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGA19770.3404529384515505No Hit
GACTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGT17970.3094557058155975No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17650.3039450866803169No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17300.29791784700110385No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17210.2963679853693062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16360.28173040329121724No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTAGTC16310.2808693690513297No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAAC15820.27243123350043136No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15660.2696759239327911No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGAGG15210.2619266157738029No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14860.2558993760945898No Hit
GTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA14470.24918330902346664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13930.23988413923268073No Hit
CTCCTGCACCGGCAGCGGTGGCAGCATTGCCACCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC13790.23747324336099548No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13600.2342013132494227No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13160.22662421193841192No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12320.21215883670830052No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGGGGTCC12170.2095757339886378No Hit
CTCTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCC11980.20630380387706496No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11600.19975994365391933No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11430.19683242723830155No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGAGATGGTGAGGGA11380.19597139299841396No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGAT11060.19046077386313345No Hit
CTTTATGTCTTCGGAACTGGGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC11030.1899441533192009No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAGG10950.18856649853538077No Hit
CTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTG10790.1858111889677405No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10670.1837447067920103No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACA10660.18357249994403277No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10240.17633981232897708No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAG10230.17616760548099955No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10220.17599539863302205No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA10220.17599539863302205No Hit
GACATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGT10020.1725512616734717No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10010.17237905482549418No Hit
GTCCTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACC10010.17237905482549418No Hit
GGAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCAT9750.16790167677807874No Hit
CCTTAGTCCCAGTTCCGAAGACATAAAGAGTGTTGCTATCATAAGACTGA9710.1672128493861687No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9660.1663518151462811No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCT9520.1639409192745959No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9310.16032457546706802No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9190.1582580932913378No Hit
CTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCT9180.1580858864433603No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9120.1570526453554952No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8940.1539529220918999No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8820.1518864399161697No Hit
CATAAAGAGTGTTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC8670.14930333719650696No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAGGACTGAGGACGAG8550.14723685502077674No Hit
ACTGAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCA8440.14534257969302408No Hit
CACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTC8430.14517037284504655No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC8330.1434483043652714No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8270.14241506327740627No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8250.14207064958145127No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8230.14172623588549624No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8210.14138182218954118No Hit
CCCCAGAGGGTCTTTCCTTATCCTCAAAGATGACATTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCA8100.1394875468617885No Hit
GCCACCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCAC8100.1394875468617885No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8090.139315340013811No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG8060.13879871946987846No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8050.13862651262190093No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA8040.1384543057739234No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT7950.13690444414212577No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG7870.13552678935830562No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA7750.13346030718257543No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG7670.1320826523987553No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT7610.1310494113108902No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC7530.12967175652707005No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7510.12932734283111502No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7370.12691644695942977No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT7330.12622761956751974No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG7300.12571099902358718No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7250.1248499647836996No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7080.12192244836808182No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA7060.12157803467212677No Hit
GGACTAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7020.12088920728021671No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7010.12071700043223918No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7010.12071700043223918No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG6970.12002817304032913No Hit
GTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTTTGAGGATA6890.11865051825650898No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC6880.11847831140853148No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG6750.11623962238482374No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATG6740.11606741553684624No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC6720.1157230018408912No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG6650.11451755390504859No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGT6620.11400093336111604No Hit
GGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACACTCTTTATGTCTTCGGAACTGGGACTAAGGTCAC6580.11331210596920596No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG6510.11210665803336335No Hit
GTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCAATGTCATCTT6510.11210665803336335No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT6500.11193445118538584No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC6490.11176224433740833No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC6490.11176224433740833No Hit
GCTGGTACCACTGCACATAGTTGGTGGCAATGCTGCCACCGCTGCCGGTG6480.1115900374894308No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG6460.11124562379347577No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6450.11107341694549826No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA6450.11107341694549826No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT6380.10986796900965565No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACAGTAACCATCTC6370.10969576216167812No Hit
GTATATTACTGTGCGAGAGATCCGGCGCGGGGAATGTGGAACTGGAACTA6280.10814590052988046No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACT6240.10745707313797041No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG6210.10694045259403787No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTCAGTCCAGA6160.10607941835415026No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT6110.10521838411426268No Hit
CCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGA6000.10332410878651001No Hit
GGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAACACT5970.10280748824257745No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA5930.1021186608506674No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5850.10074100606684724No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG5840.10056879921886974No Hit
GGCAGTGCCCCCACCAATGTCATCTTTGAGGATAAGGAAAGACCCTCTGG5820.10022438552291471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC200.006229680553.97291662
GGTATCA5000.047.597351
CGAAATT305.7224836E-446.91930453
CCGAAAT305.7831104E-446.8191152
CAGAGGG14750.039.0698270
GTGGTAT1150.038.432641
TGGTATC1200.036.8154222
GTATCAA12650.036.5513231
CCAACCG400.002464278634.86615448
AGGTCGC400.002600672434.48531740
TATCAAC13150.034.371112
ATCAACG13400.033.729863
CAACGCA13700.032.991255
TCAACGC13600.032.9839554
AATGCAG450.00370761232.0775463
AACGCAG14100.032.0553256
ACCGAAA450.004286627831.14070151
CAACCGA450.00435974831.03326249
CGCAGAG14550.030.8542758
CGCCCCA450.004507011330.82330344
Produced by FastQC (version 0.11.5)