FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546047
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGGAGTATGGTGGCTCACCTATCACTTTCGGCGGAGGGA54750.3541289495080033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44730.2893185006665386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38400.24837537280561328No Hit
CAGTAATAGATGGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATA36890.23860852871872587No Hit
GTAATAGATGGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATACA33110.21415907795817335No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32620.21098970471143505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31340.2027105256179146No Hit
GTGTATTACTGTCAGGAGTATGGTGGCTCACCTATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA31120.20128754171121577No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30720.19870029824449062No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27550.1781963937706939No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26660.17243977705723046No Hit
CTATTACTGTGCGCGAATAGCAGTGATTGGTCTCTGGTACTTTCACTACTGGGGCCAGGGAACCCTGGTCACCGT26530.17159892293054482No Hit
ATCTATTACTGTGCGCGAATAGCAGTGATTGGTCTCTGGTACTTTCACTA26280.1699818957638416No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT24430.1580158947302378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24070.1556873756101852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24010.15529928909017643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23610.15271204562345128No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23240.15031884541673055No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23100.14941331020337675No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT22950.1484430939033548No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTATC22620.14630861804330658No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22570.14598521260996594No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21820.1411341311098563No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21580.13958178502982121No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTGCT20680.13376048722968964No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20670.1336958061430215No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20270.1311085626762964No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20260.13104388158962826No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19810.12813323268956248No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19440.12574003248284174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19320.12496385944282418No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18710.12101831315606834No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18590.1202421401160508No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18590.1202421401160508No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18590.1202421401160508No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18510.11972469142270577No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTAGCTGCTGCTAACACTCTGA18290.11830170751600695No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17050.11028125276915902No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16980.10982848516248211No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16550.1070471984357526No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16420.10620634430906692No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTCTGGCATGAGCTGGGTCCGCCAGGCTCCGGGGGAGGGG16220.10491272257570436No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15950.10316633323566489No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15900.10284292780232424No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15900.10284292780232424No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15760.10193739258897046No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15480.10012632216226285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA2400.045.0965719
GACGTAG2450.042.86644426
CGAGACG2450.042.8220423
CGTAGAG2500.042.06908828
ATCTCGT1550.042.0266537
AGACGTA2500.041.98870525
CTTATAC2850.040.3456461
CGTATGC1650.039.78484341
ACGTAGA2650.039.65051327
ACGAGAC2650.039.58122322
ATGCCGT1700.038.8431744
CCGTCTT1900.038.625947
CACGAGA2750.038.13820321
GAGACGT2850.036.81669624
TCTCCGA2850.036.78573611
TCGTATG1900.036.30603440
CTAACGC952.728484E-1035.5991023
ATCTCCG2950.035.5387610
TATACAC3550.035.2481233
GTATCAA38600.034.695291
Produced by FastQC (version 0.11.5)