FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546047
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGGAGTATGGTGGCTCACCTATCACTTTCGGCGGAGGGA50580.32715693636739374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43560.28175081352636755No Hit
CAGTAATAGATGGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATA31740.2052977690846397No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30650.19824753063781372No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30640.19818284955114557No Hit
GTGTATTACTGTCAGGAGTATGGTGGCTCACCTATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA30150.1950134763044073No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30040.1943019843510579No Hit
GTAATAGATGGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATACA29640.19171474088433277No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27240.1761912800839819No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25970.16797678207712963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24990.161638035583653No Hit
ATCTATTACTGTGCGCGAATAGCAGTGATTGGTCTCTGGTACTTTCACTA24700.1597622840702773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24340.15743376495022468No Hit
CTATTACTGTGCGCGAATAGCAGTGATTGGTCTCTGGTACTTTCACTACTGGGGCCAGGGAACCCTGGTCACCGT24310.1572397216902203No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT23860.1543290727901545No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22410.14495031522327587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21590.13964646611648934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21420.13854688764313117No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21120.13660645504308733No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTATC20680.13376048722968964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20600.13324303853634464No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCTCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20600.13324303853634464No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19850.128391957036235No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGAGATGCCAGTGGCCCTGCT19150.12386428096946599No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19140.12379959988279786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18680.12082426989606396No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18470.11946596707603327No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18170.1175255344759894No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18090.11700808578264438No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17310.11196296102253037No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17200.11125146906918096No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17120.11073402037583592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17030.11015189059582275No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16900.10931103646913709No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16860.10905231212246459No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16670.10782337147577015No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTAGCTGCTGCTAACACTCTGA16650.10769400930243389No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTCTGGCATGAGCTGGGTCCGCCAGGCTCCGGGGGAGGGG16590.10730592278242511No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16360.10581825778905816No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16170.10458931714236372No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15900.10284292780232424No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15860.10258420345565172No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15490.10019100324893099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)