FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331866
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCAACACCACCCAGGCATAGCCCCCAAACTCATGATTTATGAGGTCA58710.44081011152773625No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC52510.3942588818995304No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAAT50490.3790921909561472No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48560.36460124366865737No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGCTGCTGGTTGTATATGAGCTG42340.31789984878358635No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAACTCAGCATCAGGGAGAACTGGTTCTTGGACGTGTCTATTGA36420.273450932751493No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35540.26684366144942506No Hit
GTGTTGGTACCACGAGACATAATTATAAGCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA35330.2652669262523407No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34120.2561819282119973No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG33940.2548304409002107No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33620.2524277967903678No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33320.2501753179373901No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32740.24582052548829986No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32430.24349296400688955No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT28700.2154871436015335No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28500.21398549103288167No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27350.20535098876313385No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27240.20452507985037532No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCACTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT26930.20219751836896505No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC26700.20047061791501544No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG26360.1979178085483074No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26120.19611582546592526No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26030.19544008181003195No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25490.19138561987467206No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTCTCGTGGTACCAACACCACCCAGGCATAGC25260.18965871942072252No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24460.1836521091461153No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24310.18252586971962645No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24030.18042355612351393No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23110.17351595430771563No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22760.17088806231257497No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22530.16916116185862543No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21660.16262897318499006No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21460.16112732061633828No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGCTGCTGG21320.16007616381828202No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21240.15947550279082132No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20680.15527087559859626No Hit
CCCAGGCATAGCCCCCAAACTCATGATTTATGAGGTCAGAAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGG20630.15489546245643332No Hit
GGCATAGCCCCCAAACTCATGATTTATGAGGTCAGAAATCGGCCCTCAGG20360.15286823148875336No Hit
GATTATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCT20110.1509911657779386No Hit
CCCAAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCC20050.15054067000734309No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAATTATGTCTCGTGGTACCAACACCA19850.14903901743869127No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATAATCAGGGAGG19820.1488137695533935No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19650.14753736487003946No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18970.14243174613662335No Hit
GTGTATTACTGTGCGAGAGGGGCATATTGTACTACTGGTACCTGCCACTT18370.13792678843066794No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17810.13372216123844288No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17620.13229559129822369No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17380.13049360821584152No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGA16740.12568831999615576No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16580.12448699794123433No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16520.12403650217063877No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16490.12381125428534102No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16420.12328567588631288No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16250.12200927120295886No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15990.12005712286371152No Hit
ATGCCTGGGTGGTGTTGGTACCACGAGACATAATTATAAGCACCAACGTC15890.1193062965793856No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15800.1186305529234923No Hit
GAGATAATCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT15660.11757939612543605No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15530.11660332195581238No Hit
CCTTGGTCCCTCCGCCGAACACCCAAGTGCTGCTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAG15480.11622790881364942No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC15460.11607774355678424No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15340.11517675201559315No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15250.11450100835969985No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15170.11390034733223912No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15140.11367509944694137No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14730.11059671168120516No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14700.1103714637959074No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14560.10932030699785113No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14560.10932030699785113No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14360.10781865442919933No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14180.10646716711741272No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14140.10616683660368234No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14020.10526584506249127No Hit
GCCTGGAGCCCAGAGATAATCAGGGAGGCCGTGTTGCCAGACTTGGAGCC13550.10173696152615953No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13410.10068580472810329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)