FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273281
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42700.33535409701393487No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42510.33386188908811176No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA39690.3117143819785263No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33880.26608423435204015No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33090.25987979087098606No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32880.25823050842665524No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC32350.25406803368620123No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31900.25053385701977804No Hit
GATTATTACTGCAGCTCATATAGAAGCAGCAACACTCTCAATGTCTTCGG31750.2493557981309703No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGAGAGTGTTG30710.24118792316857002No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27100.21283597257793055No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT25980.2040397995414995No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25680.20168368176388402No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24790.19469386569029146No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT24760.1944582539125299No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24680.1938299558384991No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24540.19273043420894523No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC23860.1873899005796835No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA22040.173096119395483No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21950.1723892840621984No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21850.17160391146965986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21830.17144683695115218No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC21560.16932633095129826No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21380.16791266028472898No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20860.16382872280352884No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20050.1574672048039671No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19730.1549540125078439No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCCGATCATTTGCAGATACA18910.1485139572490283No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18750.1472573611009667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18650.14647198850842824No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT18620.14623637673066667No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTGAGAGTGTTGCTGCTTCTATATGAG18150.14254512554573578No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17990.1412885293976742No Hit
CTCTCAATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC17700.1390109488793126No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16970.13327772895378162No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16200.12723035999123525No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15790.12401033236182743No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT15530.12196836362122737No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15250.1197693203621196No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15070.1183556496955503No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15060.11827711243629646No Hit
GTATATTACTGTGCGAAAGGGTTCTGGCTTCGCCATGGCAGCCCGTCGCCAACTGACTACTGGGGCCAGGGAACC14790.11615660643644254No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14710.11552830836241176No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14590.11458586125136558No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14360.11277950428852704No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14150.11113022184419621No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14080.11058046102941928No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13990.10987362569613464No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13950.10955947665911923No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCT13510.10610383725194988No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG13290.1043760175483652No Hit
CATTGAGAGTGTTGCTGCTTCTATATGAGCTGCAGTAATAATCAGCCTCG13270.10421894302985751No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13250.10406186851134981No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13020.10225551154851129No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12950.10170575073373433No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12760.10021354280791123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)