FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962875
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37980.39444372322471766No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA32070.333065039594963No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGTTACCA31390.32600285603011814No Hit
CCCCTGTACTTGTCATCTATGGTAGCACCAACCGGCCCTCAGGGATCCCA29460.30595871738283786No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29130.30253148124107493No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC27280.28331818771907047No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27090.2813449305465403No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26760.27791769440477737No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26400.27417889134103596No Hit
GTACAGGGGCCTGTCCCGGCTTCTGCTGGTACCAGGTTGCATAATAGCTT25100.26067765805530313No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG23210.24104894197066076No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23110.24001038556406595No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21720.22557445151239777No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21710.2254705958717383No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21650.2248474620277814No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21090.21903154615085033No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20340.2112423731013891No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19650.20407633389588473No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19460.20210307672335454No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19450.20199922108269505No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19430.2017915098013761No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19200.19940283006620796No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18410.1911982344541088No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17130.1779047124496949No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17050.17707386732441907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16960.1761391665584837No Hit
GTAATATACGGCCGTATCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16710.17354277554199662No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16640.17281578605738024No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16300.16928469427495782No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGACCTGACTCAGGACCCTGCT15970.16585745813319486No Hit
CCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT15820.1642996235233026No Hit
TGGTAGCACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGAC15820.1642996235233026No Hit
CTATTACTGTAACTCCCGGGACTCCCGGGACAGCAGTGGTAACCATTGGGTGTTCGGCGGAGGGACCAAGCTGAC15720.16326106711670776No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15620.16222251071011293No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGTTACCAC15520.16118395430351812No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGACCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA15250.15837985200571206No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14850.15422562637933274No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGACCTGACTCAGGA14040.14581331948591458No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13980.14519018564195768No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13410.13927041412436714No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA13340.13854342463975075No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13260.13771257951447488No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13210.13719330131117746No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12940.13438919901337143No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12930.13428534337271192No Hit
CTATTATGCAACCTGGTACCAGCAGAAGCCGGGACAGGCCCCTGTACTTGTCATCTATGGTAGCACCAACCGGCC12600.13085810723094898No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12510.12992340646501363No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12500.12981955082435415No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12470.1295079839023757No Hit
ACCTGGTACCAGCAGAAGCCGGGACAGGCCCCTGTACTTGTCATCTATGGTAGCACCAACCGGCCCTCAGGGATC12440.12919641698039724No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAACCTGGTA12360.12836557185512137No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12170.1263923146825912No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12150.12618460340127224No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11880.12338050110346618No Hit
GGTTACCACTGCTGTCCCGGGAGTCCCGGGAGTTACAGTAATAGTCAGCC11870.12327664546280669No Hit
AGCCGGGACAGGCCCCTGTACTTGTCATCTATGGTAGCACCAACCGGCCC11720.12171881085291446No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11720.12171881085291446No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11710.12161495521225496No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11670.12119953264961704No Hit
GTGCTACCATAGATGACAAGTACAGGGGCCTGTCCCGGCTTCTGCTGGTACCAGGTTGCATAATAGCTTCTGAGG11600.12047254316500065No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11220.11652602881994029No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11170.11600675061664287No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG10860.11278722575619889No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10700.11112553550564715No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC10610.1101908347397118No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT10290.10686745423860833No Hit
ATGGTAGCACCAACCGGCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAGCTTCCTTGA10230.10624432039465144No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10180.10572504219135402No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10130.1052057639880566No Hit
GTGGTAACCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10020.1040633519408023No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9980.10364792937816435No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9860.10240166169025054No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAC9740.10115539400233674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT200.007693394651.17099413
GGTATCA8100.042.962831
TCGTATG758.62201E-1041.44258540
GTGGTAT2700.040.4356041
GTATCAA23100.040.0253371
GGATGGG6450.039.03078570
CGGGTAT702.4179826E-838.985417
CGTATGC801.5952537E-938.92241
TCTGGAG6900.038.40557570
GCTAATC655.7540274E-736.89719833
TCTCGTA754.087633E-836.72971338
TGGTATC3000.036.3920442
TATCAAC25550.035.7866942
CCGGTTA601.3599985E-534.43410538
AATCTCG807.3734554E-834.3476836
ATCAACG26950.034.1808363
TATACAC1400.034.1175423
ACGAGAC1103.274181E-1134.11222522
TCAACGC27450.033.5582354
CAACGCA27600.033.499475
Produced by FastQC (version 0.11.5)