FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947931
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGA56950.6007821244373271No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38140.40234996007093343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37190.3923281335877822No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32340.34116407206853666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28390.29949437248069744No Hit
GTGTATTACTGTCAGCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26910.2838814217490514No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25730.27143325832787407No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23970.2528665061064571No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23770.25075664789947794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23370.2465369314855195No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22440.23672609082306625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22210.23429975388504018No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21930.23134595239526928No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21670.2286031367261963No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATT20520.21647145203606588No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGATAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18860.19895962891813856No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18720.1974827281732531No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18110.19104766064196654No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17320.1827137207243987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16990.17923245468288304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16600.17511823117927358No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16490.17395780916543505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16100.1698435856618256No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTTTTACCAG15260.16098218119251295No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGGTAAAACTCTGA15040.15866133716483583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14580.15380866328878368No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14500.15296472000599198No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14290.15074936888866383No Hit
GTCCCAGACCCACTGCCACTGAACCTATCTGGGATGCCAGTGGCCCTCCT14230.1501164114265701No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13860.14621317374365855No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGA13850.1461076808333096No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13820.14579120210226273No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13630.14378683680563248No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13340.14072754240551263No Hit
GACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGGTAGCTCA13170.1389341629295803No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12970.13682430472260113No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12820.13524191106736672No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12790.13492543233631984No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12720.13418698196387713No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12640.13334303868108544No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12440.13123318047410623No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12290.12965078681887182No Hit
GTGTAAAGTGAGCTACCATAGTGCTGACAGTAATACACTGCAAAATCTTC12140.12806839316363744No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12140.12806839316363744No Hit
GCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT11980.12638050659805408No Hit
ATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11970.1262750136877051No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11630.12268825473584048No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11600.12237177600479361No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11570.12205529727374671No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11290.11910149578397584No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11240.11857403123223104No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11200.11815205959083519No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11110.11720262339769455No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11070.11678065175629872No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10960.11562022974246015No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10920.11519825810106432No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10890.11488177937001745No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10740.11329938571478304No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTATCTGGGATGCCA10730.11319389280443407No Hit
ATCCAGGAGGGCCACTGGCATCCCAGATAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATCAG10580.11161149914919968No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10530.11108403459745488No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10360.10929065512152256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10320.10886868348012671No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10270.10834121892838193No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10200.1076027685559392No Hit
GAGTTTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10190.10749727564559025No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9970.10517643161791312No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9930.10475445997651728No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9690.10222263012814223No Hit
CTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9650.10180065848674641No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9640.10169516557639745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAAA305.5232405E-447.26099457
GGTATCA10800.045.35381
ATCTCGT1150.044.824237
CGTATGC1350.043.5766341
TCGTATG1450.042.88424740
GTATCAA30700.040.3309021
ATGCCGT1500.039.3626144
ACGAGAC1950.038.4046622
TCTCCGA2100.037.25490611
AACTGTG23500.036.52610470
CGAGACC2200.035.59342223
ATCTCCG2200.035.561510
TATCAAC34900.035.477332
CCGAAAC450.00256696934.5789968
ATGTCGT601.3787516E-534.36522337
CATCTCG1500.034.32863236
ATCAACG36500.033.8289643
TCAACGC36800.033.4607544
CAACGCA37050.033.3267825
AACGCAG37600.032.7488256
Produced by FastQC (version 0.11.5)