FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947931
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGA53310.5623827050703057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37690.3976027791052303No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30190.31848309634351024No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26390.2783957904109054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25880.2730156519831085No Hit
GTGTATTACTGTCAGCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25020.2639432616930979No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24700.2605674885619312No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23410.24695890312691535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22520.23757003410585792No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21140.22301201247770142No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20770.21910877479478993No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19540.2061331468218678No Hit
GTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATT19150.20201892331825838No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18030.19020371735917488No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGATAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17400.1835576640071904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17210.18155329871056017No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGATGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17120.18060386251741953No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16660.17575118864136735No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16510.17416879498613297No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16220.1711095005860131No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16070.16952710693077871No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15840.16710076999275264No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15540.16393598268228385No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15450.16298654648914318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14770.15581302858541393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14230.1501164114265701No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTTTTACCAG13930.14695162411610127No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13590.14336486516423663No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13270.13998909203306992No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13230.13956712039167407No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGA13180.13903965583992928No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGGTAAAACTCTGA13110.13830120546748656No Hit
GTCCCAGACCCACTGCCACTGAACCTATCTGGGATGCCAGTGGCCCTCCT13110.13830120546748656No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12900.1360858543501584No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12800.1350309252466688No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12760.13460895360527295No Hit
GACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGGTAGCTCA12380.13060022301201246No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12150.12817388607398641No Hit
ATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11790.12437614130142384No Hit
GTGTAAAGTGAGCTACCATAGTGCTGACAGTAATACACTGCAAAATCTTC11360.11983994615641855No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11110.11720262339769455No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10640.11224445661129342No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTATCTGGGATGCCA10630.11213896370094448No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10620.11203347079059552No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10610.11192797788024655No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10520.11097854168710593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10460.11034558422501216No Hit
GAGTTTTACCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG10350.10918516221117361No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10330.10897417639047567No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10310.10876319056977776No Hit
GCACTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10250.10813023310768399No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10190.10749727564559025No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9920.10464896706616832No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9870.10412150251442352No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9820.10359403796267871No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9810.10348854505232977No Hit
CTATGGTAGCTCACTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT9740.10275009467988704No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9610.10137868684535055No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9560.10085122229360577No Hit
ATCCAGGAGGGCCACTGGCATCCCAGATAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATCAG9550.1007457293832568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10950.044.4002721
GTATCAA30750.039.471791
CGGGATT451.1017666E-437.7166676
CACCGAA400.002451210534.90628448
TATATTC601.4977781E-533.9521643
TATCAAC35100.033.8786772
ATCAACG36300.032.829783
TCAACGC36650.032.4167824
CAACGCA37300.031.9428835
GACAGGT1500.031.7322241
AACGCAG38000.031.443796
AACTGTG23250.031.10803470
AGAGTAC39600.030.33997511
CGCAGAG39800.030.1922878
CTAACGC450.004998685330.1797033
ACGCAGA40150.029.9290927
GCAGAGT40800.029.6171159
GCTTATA605.9060816E-428.3323421
CAGAGTA43950.027.57016610
TGTTAGA12100.026.18657570
Produced by FastQC (version 0.11.5)