FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916200
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCT29790.32514734774066795No Hit
GTGTTGATACCAGGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAG29390.3207814887579131No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28300.30888452302990616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28160.30735647238594194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23970.2616240995415848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21780.23772102161100195No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18870.2059593975114604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18460.20148439205413662No Hit
ACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT18210.19875573018991485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17480.19078803754638723No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16630.18151058720803318No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16620.18140144073346431No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16550.1806374154114822No Hit
GGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT16230.17714472822527833No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15820.1726697227679546No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAATTTGATTCTTAT15730.17168740449683476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15610.1703776468020083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14970.16339227242960053No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14830.1618642217856363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14450.1577166557520192No Hit
AGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGACCGGGGAA13990.1526959179218511No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT13910.15182274612530017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13860.1512770137524558No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13860.1512770137524558No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCCCTCCGCCGAAAGTG13840.15105872080331806No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTGCCAGTGCTTTAGCCTGGTATCAACACAGA13400.1462562759222877No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13290.14505566470203013No Hit
ACTTATTACTGTCAACAATTTGATTCTTATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAA13250.14461907880375463No Hit
GCTTTAGCCTGGTATCAACACAGACCGGGGAAAGCTCCTAAGCTCCTGAT13090.1428727352106527No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12430.13566906788910718No Hit
GATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGAT12050.13152150185549008No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11280.12311722331368696No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG11250.12278978388998035No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11240.12268063741541148No Hit
GACCCACTCAGGACACAGCTTGGACATGAGGGTCCCCGCTCAGCTCCTGG11150.12169831914429165No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11070.12082514734774066No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCGCTGCCG11060.12071600087317179No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10820.11809648548351888No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10600.1156952630430037No Hit
GCCTTATATTACTGTGCGAAAGGCGAGAGATCATCTATAGAGACTGCCATGGGTTACTACTTTGACTTTTGGGGC10460.1141672123990395No Hit
CATTAGGATAAGAATCAAATTGTTGACAGTAATAAGTTGCAAAATCTTCA10450.11405806592447064No Hit
GCTTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG10250.1118751364330932No Hit
ATCCTAATGGGCTCACTTTCGGCGGAGGGACCCAGGTGCAGATCAAACGA10080.11001964636542241No Hit
GAGTGAAGTCTGTCCCAGATCCGCTGCCGCTGAACCTCGATGGGACTCCA10040.1095830604671469No Hit
GTGGAGTCCCATCGAGGTTCAGCGGCAGCGGATCTGGGACAGACTTCACT10010.10925562104344029No Hit
CCCAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9930.10838244924688933No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9920.10827330277232046No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9910.10816415629775158No Hit
GTAATATAAGGCCGTGTCGTCGGCTCTCAGGGTGCTCATCTGCAGATAGA9880.10783671687404497No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTGGGTCC9800.10696354507749399No Hit
GGCTAAAGCACTGGCAATGCCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGA9770.10663610565378738No Hit
ACACAGACCGGGGAAAGCTCCTAAGCTCCTGATCTTTGATGCCTCCACTTTGGAAAGTGGAGTCCCATCGAGGTT9730.1061995197555119No Hit
CTCCTGTGGTGCCTCTGGATTCACTTTTAGCGCCTATGCCATGACCTGGGTCCGCCAGGCTCCCGGGAAGGGG9570.10445317616240996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9360.10216110019646366No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9340.1019428072473259No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9210.10052390307793058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9170.10008731717965509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT554.3655746E-1156.05510339
CGTATGC3850.045.74785241
ATGCCGT4150.045.42411444
CTTATAC4250.045.1214331
TCGTATG3850.044.6624340
CCGTCTT4100.043.01347447
TATACAC4650.041.9510543
TTATACA4600.041.688282
CGTCTTC4200.041.3421148
TACACAT4900.041.1624575
TGTCGTA759.713403E-1040.94731538
GTATGCC4500.040.8283442
ATGTCGT751.0040822E-940.80481737
ATCTCCG4550.040.6952910
ATACACA5300.039.3014454
ATCTCGT3650.039.12790337
CTTGAAA5100.039.09145757
ACGAGAC5000.038.3846422
TTGAAAA5350.038.14079358
TGCTTGA4600.038.12758355
Produced by FastQC (version 0.11.5)