FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890371
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49400.5548248988343062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47380.5321377268576807No Hit
AGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTTCTCATCGGGCC36710.41230004121877284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34220.38433417081194243No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30140.3385105759284613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30010.3370505104052131No Hit
GTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA28070.315261840289048No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27850.3127909601727819No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27850.3127909601727819No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTC26210.29437167203334336No Hit
GTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTT25820.2899914754635989No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25810.2898791627310413No Hit
GTCCTGGCTTCTGCAGGTACCAACCCAAATAGCTGTTTCCATTACTATGC25230.2833650242427033No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24940.2801079549985343No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24710.2775247621497106No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24490.2750538820334445No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24430.27438000563809917No Hit
CACCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG23810.26741661621953094No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23460.2634856705800166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22240.2497835172079953No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATT21660.24326937871965731No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT21040.23630598930108912No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20630.23170116726622947No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20350.22855641075461802No Hit
GTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA19920.22372696325464328No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19880.22327771232441307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19440.21833595209188078No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA19110.21462963191748158No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT18920.2124956899988881No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18640.20935093348727665No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18030.202499856801266No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA17720.19901816209198187No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17340.1947502782547949No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17320.19452565278967981No Hit
GTCTCCACTCTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCT17180.1929532745338741No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACTCTCC16890.18969620528970507No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG16820.18891001616180222No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16800.1886853906966871No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16770.18834845249901447No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGA16390.18408056866182748No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG16160.1814973758130038No Hit
GTCAGGGACCCCGGAGGCCCGATGAGAACCCAAATAGATCAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA16100.18082349941765846No Hit
CTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG15850.17801568110371968No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACAC15710.17644330284791396No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15570.17487092459210823No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15120.1698168516270184No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14900.16734597151075226No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14760.16577359325494653No Hit
TTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGA14300.16060720755729915No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACT14090.15824864017359055No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAATTCGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGGCT14050.15779938924336034No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14020.1574624510456877No Hit
CTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA13950.15667626191778483No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13950.15667626191778483No Hit
CAGTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13880.15589007278988198No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13610.15285762901082808No Hit
GAATTCGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAAT13470.15128525075502236No Hit
GAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGA13300.14937593430154397No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13190.14814049424341091No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13140.14757893058062313No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13110.1472419923829505No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13090.14701736691783537No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12870.14454648680156923No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12860.14443417406901168No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12830.14409723587133902No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12750.14319873401087863No Hit
AGTCAGAGCCTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAG12670.1423002321504182No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12660.14218791941786063No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12480.14016629023182473No Hit
CTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTGAT12110.13601071912719528No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12080.13567378092952262No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11950.13421371540627444No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11920.1338767772086018No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11910.13376446447604426No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11880.1334275262783716No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTCATCGGGCCTCCGGGGTCC11470.12882270424351197No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11420.12826114058072421No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11370.12769957691793646No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGTGTGACGGGCAGG11250.12635182412724583No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11240.12623951139468828No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11160.12534100953422786No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA11100.12466713313888256No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11070.12433019494120989No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATA10980.12331938034819193No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10910.12253319122028908No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10780.1210731256970409No Hit
CCACTCTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCTGCAG10700.12017462383658048No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10650.11961306017379272No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10490.11781605645287188No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10180.11433436174358778No Hit
GCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGAC10170.11422204901103024No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10160.11410973627847268No Hit
GGCCGAAGGTGATCGGAATTCGTAGAGCTTGCATGCAGTAATAAACCCCA10140.11388511081335759No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10130.11377279808080001No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10070.11309892168545471No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9890.11107729249941879No Hit
GGGATACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCTGCAGGT9760.10961722697617061No Hit
ATTTGGGTTCTCATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT9740.1093926015110555No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9720.10916797604594039No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9690.10883103784826774No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTC9270.10411390308085057No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAACCCAAATAGCTGTTTCCA9260.10400159034829302No Hit
GGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA9180.1031030884878326No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTC9090.10209227389481465No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT8960.10063220837156646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAA150.002467351368.1964523
ATATCGT200.007441856451.60271537
ATCTCCG3400.043.087910
CGTATGC3450.043.0828441
TCGTATG3450.043.038740
ATGCCGT3500.042.64963544
CGAGACG3450.042.49923723
GACGGAC3400.042.1616226
GTATGCC3550.041.92657542
ATCTCGT3300.041.6991637
CTTATAC3700.041.43821
GGTATCA8750.041.2750851
TATCTCG3250.041.25409736
ACGAGAC3600.040.7169922
TTATACA3700.040.515082
CGGACTC3550.040.43474228
TCCGAGC3650.040.13892713
TCTCCGA3750.039.9748811
CACGAGA3700.039.6120821
TCTTATA955.456968E-1239.4488952
Produced by FastQC (version 0.11.5)