FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890371
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48890.549096949473871No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38630.43386408586982284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36780.4130862303466757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35000.3930945639514315No Hit
AGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTTCTCATCGGGCC34390.3862434872654208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29500.331322561044778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28660.3218882915099436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28460.31964203685879256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26880.3018966251146994No Hit
GTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA26860.30167199964958424No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTC26130.293473170172883No Hit
GTCCTGGCTTCTGCAGGTACCAACCCAAATAGCTGTTTCCATTACTATGC24190.27168450005671796No Hit
GTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTT24160.27134756185904524No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24150.2712352491264877No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23410.2629241069172289No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22810.25618534296377576No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22360.2511312699986859No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22360.2511312699986859No Hit
CACCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG21990.24697569889405654No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21860.24551563337080834No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21490.24136006226617895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21420.24057387313827605No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATT21300.23922612034758545No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20890.23462129831272582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20380.2288933489522907No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19740.22170533406860737No Hit
GTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA19300.21676357383607509No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18930.21260800273144564No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18130.20362298412684152No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG18080.20306142046405376No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17910.20115210401057537No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17790.1998043512198847No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.19890584935942432No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17540.19699653290594596No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16890.18969620528970507No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACTCTCC16320.1832943795339246No Hit
GTCTCCACTCTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCT16050.18026193575487073No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15820.17767874290604702No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15440.17341085906886006No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15140.1700414770921335No Hit
GAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGA15100.1695922261619033No Hit
CTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG15050.16903066249911552No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA14920.16757059697586735No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14900.16734597151075226No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAATTCGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGGCT14880.16712134604563716No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14680.1648750913944861No Hit
GTCAGGGACCCCGGAGGCCCGATGAGAACCCAAATAGATCAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA14640.1644258404642559No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14550.16341502587123793No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGA14440.16217958581310488No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACAC14400.16173033488287467No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14190.15937176749916607No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13900.15611469825499708No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13470.15128525075502236No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13430.15083599982479212No Hit
CTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA13330.14971287249921664No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13210.14836511970852598No Hit
TTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGA13200.14825280697596843No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13070.14679274145272025No Hit
CAGTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12990.14589423959225986No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12780.14353567220855126No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATACTGTGATGACTCAGTCTCCACT12670.1423002321504182No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12600.14151404302251533No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12460.1399416647667096No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12400.1392677883713643No Hit
GAATTCGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAAT12290.13803234831323122No Hit
AGTCAGAGCCTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAG12210.13713384645277082No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12080.13567378092952262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12080.13567378092952262No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11650.1308443334295479No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11630.1306197079644328No Hit
CTCCTGCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTGAT11600.13028276976676015No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11570.1299458315690875No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11460.12871039151095442No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11430.1283734533132818No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11410.12814882784816667No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11300.12691338779003358No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11230.12612719866213074No Hit
GGCCGAAGGTGATCGGAATTCGTAGAGCTTGCATGCAGTAATAAACCCCA11210.12590257319701564No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11150.12522869680167031No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTCATCGGGCCTCCGGGGTCC11130.12500407133655522No Hit
GTGGGGATACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACACCT11070.12433019494120989No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11040.12399325674353724No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10660.11972537290635028No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10620.11927612197612006No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10600.11905149651100495No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGTGTGACGGGCAGG10550.11848993284821721No Hit
GGGATACTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACACCTGGAGAGCCGGCCTCCATCTCCTGCAGGT10460.11747911825519923No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA10350.11624367819706616No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATA10330.11601905273195107No Hit
GGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA10320.11590673999939351No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10190.11444667447614534No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10170.11422204901103024No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10170.11422204901103024No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10130.11377279808080001No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10020.11253735802266696No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9930.11152654342964899No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9580.10759559779013467No Hit
GCATAGTAATGGAAACAGCTATTTGGGTTGGTACCTGCAGAAGCCAGGAC9540.10714634685990447No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9470.1063601577320016No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9390.10546165587154117No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9310.10456315401108078No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAACCCAAATAGCTGTTTCCA9270.10411390308085057No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9210.10344002668550525No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACGAATTCCGATCACCTTC9200.10332771395294771No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG9020.10130608476691177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAT200.00778992751.0095215
GGTATCA10300.047.8864671
GTGGTAT1900.037.5965231
GTATCAA22400.034.167761
ATTCACT1900.032.218352
TATAATA450.00434253731.06020748
TCGAACT450.00496651730.2193764
ATCAACG26250.029.0154913
CGGTACC605.168832E-428.98508845
TGGTATC2350.028.94322
TCAACGC26650.028.702744
TATCAAC26650.028.5848052
CAACGCA26900.028.5623665
AACGCAG27250.027.9475616
CCGTGAA905.069307E-627.92070862
TATACAT2803.092282E-1127.8796270
CACTTTA2150.026.881195
GAGTTTA1900.026.854661
ACGCAGA28750.026.489437
AGAGTAC28500.026.48320411
Produced by FastQC (version 0.11.5)