FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971867
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52350.5386539516209522No Hit
GATTAATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCAT51940.5344352673771205No Hit
GTATTAATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC46810.4816502669603968No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46480.4782547406178006No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37600.3868842135806648No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGATTCAGGGAGAACTGGTTCTTAGACTTCTCAACTGA33600.34572631851889196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32340.33276158157443353No Hit
GTAGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAG30130.310021844552804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29810.3067292129478622No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29500.3035394760805748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29240.30086421290155957No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28490.29314710757747714No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25640.263822107345964No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25580.2632047389200374No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24960.2568252651854626No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGCG24850.25569342307126386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24540.25250368620397645No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24510.2521950019910132No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTT24120.24818210722249032No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23360.24036210716075349No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23180.2385100018829737No Hit
GATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22630.23285079131197994No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22330.22976394918234697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22320.2296610544446925No Hit
CTACTACACTGGGTACACCAACTACAACCCGTCCCTCAAGAGTCGACTTACCATGTCAGTTGAGAAGTCTAAGAA21500.2212236859570291No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21200.21813684382739612No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT20730.21330079115763784No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20120.20702421216071748No Hit
ACATAGATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGC20050.20630394899713642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20050.20630394899713642No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18750.19292763310206026No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18250.18778289621933866No Hit
GTGTATTACTGTGCGAGAACAGTGGAGTCGGAGACTTATACTTATTTTTACTTTGACTACTGGGGCCAGGGAACC17980.18500473830266898No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17660.18171210669772714No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17460.17965421194463851No Hit
CTGTAGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGC17450.17955131720698408No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17040.17533263296315235No Hit
GTGTAGTAGATGGAGCCAATCCACTCCAGGCCCTTTCCTGGGAGCTGACG16350.16823289606499656No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16150.1661750013119079No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15860.16319105391992939No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15610.16061868547856856No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15600.16051579074091413No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGATTAATACTTAC15450.15897236967609765No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15190.15629710649708242No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC14560.14981473802485318No Hit
ACTTATTACTGTCAACAGATTAATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAA14560.14981473802485318No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14490.14909447486127217No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14380.1479626327470734No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13660.1405542116359543No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13580.13973105373471884No Hit
ATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA13470.1385992116205201No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13330.13715868529335803No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCTGCTCCCAGATGGGTC13320.1370557905557036No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13250.1363355273921226No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13230.13612973791681374No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13190.135718158966196No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13140.13520368527792384No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13130.1351007905402694No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12930.13304289578718076No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12920.13294000104952633No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12870.13242552736125415No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGATTCAGGGAGAACTGGTTCTTAGACTTCTCAA12690.1305734220834744No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12680.13047052734581996No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAACATAGATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTG12650.13016184313285667No Hit
GTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTGAACTCCTGATCTATGTTG12600.1296473694445845No Hit
CTCCTGATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTT12600.1296473694445845No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12490.12851552733038574No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12440.1280010536421136No Hit
ATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGA12410.1276923694291503No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12310.12666342205260597No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG12300.12656052731495154No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12280.12635473783964266No Hit
GGGTACACCAACTACAACCCGTCCCTCAAGAGTCGACTTACCATGTCAGTTGAGAAGTCTAAGAACCAGTTCTCC12270.12625184310198823No Hit
TCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT12170.1252228957254439No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12150.12501710625013504No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCC12050.12398815887359071No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12000.12347368518531857No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11890.12234184307111981No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11770.12110710621926664No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11730.1206955272686489No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11690.12028394831803117No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG11670.12007815884272231No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11590.11925500094148685No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11530.11863763251556025No Hit
GTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAATAATTGCTA11390.11719710618839821No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11380.11709421145074378No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11360.1168884219754349No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11200.11524210617296399No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11190.11513921143530956No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11080.1140073693211108No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11010.1132871061575298No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10930.11246394825629434No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10920.1123610535186399No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10920.1123610535186399No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10890.11205236930567661No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10720.11030315876555126No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAT10720.11030315876555126No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10540.10845105348777147No Hit
GGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTGAACTCCTGATCTATGTTGCGTC10510.10814236927480818No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10390.10690763242295498No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10290.10587868504641067No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10220.10515842188282966No Hit
GCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGCGGGAGG10150.10443815871924864No Hit
CCCCTGAACTCCTGATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCA10120.10412947450628533No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10060.10351210608035874No Hit
ATCTACTACACTGGGTACACCAACTACAACCCGTCCCTCAAGAGTCGACT10000.10289473765443215No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9820.10104263237665236No Hit
GGTAAGTATTAATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT9790.10073394816368907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTA150.002487717268.0556820
CTAACGC200.00777703551.0312543
CAACTGA9850.040.19751469
TGGACCG451.08740765E-437.8009345
GTGGTAT1850.036.7792851
TCTCAAC9450.035.3704666
AGTTCGA400.00231654935.31074555
AATGGTA4050.034.85761670
GTATCAA16650.034.7359921
ACGGGAC951.0077201E-832.2302673
AGTTTAT2900.031.6745722
ATCAACG18500.031.6301843
TATCAAC18650.031.1933672
AGCCGTT450.004360489531.03432549
TCAACGC18800.030.9444874
ATACGTA553.5306925E-430.934411
TACGTAC553.5306925E-430.934412
GAGTTTA3000.030.6187551
TTAGACT11250.030.35880359
AACGCAG19250.030.2242166
Produced by FastQC (version 0.11.5)