FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971867
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50130.5158113198616683No Hit
GATTAATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCAT48060.4945121091672009No Hit
GTATTAATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC44100.45376579305604575No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40520.41692947697575905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36090.37134710819484557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33910.34891605538617937No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGATTCAGGGAGAACTGGTTCTTAGACTTCTCAACTGA30510.31393184458367246No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29960.3082726340126787No Hit
GTAGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAG28860.2969542128706911No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28840.29674842339538227No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25530.26269026523176525No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25470.2620728968058387No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24590.25301815989224863No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTT24060.2475647387965637No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23610.24293447560211429No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGCG23450.24128815979964335No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22860.23521737027803186No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22820.23480579132741414No Hit
GATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22800.23460000185210528No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22790.23449710711445088No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22290.22935237023172925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22190.22832342285518492No Hit
CTACTACACTGGGTACACCAACTACAACCCGTCCCTCAAGAGTCGACTTACCATGTCAGTTGAGAAGTCTAAGAA21290.21906289646628604No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21050.21659342276257967No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20240.20825894901257067No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20140.20723000163602634No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18980.1952942120681122No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18850.1939565804786046No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18830.19375079100329573No Hit
GTGTATTACTGTGCGAGAACAGTGGAGTCGGAGACTTATACTTATTTTTACTTTGACTACTGGGGCCAGGGAACC18350.188811843595883No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17950.18469605408970569No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT17890.1840786856637791No Hit
ACATAGATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGC17700.18212368564834489No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17360.1786252645680942No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17270.1776992119292043No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16540.17018789608043075No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16520.1699821066051219No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16460.1693647381791953No Hit
GTGTAGTAGATGGAGCCAATCCACTCCAGGCCCTTTCCTGGGAGCTGACG16150.1661750013119079No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16120.16586631709894462No Hit
CTGTAGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGC16030.16494026446005472No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGATTAATACTTAC15160.1559884222841191No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14780.1520784222532507No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14700.15125526435201525No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14490.14909447486127217No Hit
ACTTATTACTGTCAACAGATTAATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAA14090.1449786853550949No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13970.1437439485032417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13970.1437439485032417No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC13830.14230342217607964No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13820.1422005274384252No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCTGCTCCCAGATGGGTC13810.14209763270077078No Hit
CTCCTGATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTT13790.14189184322546192No Hit
ATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA13500.1389078958334834No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13500.1389078958334834No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13430.13818763266990236No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13120.13499789580261495No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12980.1335573694754529No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12960.13335158000014405No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGATTCAGGGAGAACTGGTTCTTAGACTTCTCAA12860.13232263262359972No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12750.13119079050940097No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12730.1309850010340921No Hit
GGGTACACCAACTACAACCCGTCCCTCAAGAGTCGACTTACCATGTCAGTTGAGAAGTCTAAGAACCAGTTCTCC12620.12985315891989338No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12600.1296473694445845No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12440.1280010536421136No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12330.12686921152791483No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12320.1267663167902604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12250.12604605362667937No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG12240.12594315888902494No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGACGCAACATAGATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTG12210.12563447467606165No Hit
GTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTGAACTCCTGATCTATGTTG12160.12512000098778947No Hit
ATACTTACCCTCCCGCTTTCGGCCCTGGGACCAAAGTGGATTTCAAACGA11900.12244473780877424No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11890.12234184307111981No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11660.11997526410506788No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11620.11956368515445015No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11490.11822605356494253No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11400.11730000092605264No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG11340.11668263250012605No Hit
GTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAATAATTGCTA11220.11544789564827285No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11210.11534500091061842No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11110.11431605353407409No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11070.11390447458345637No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11070.11390447458345637No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10970.11287552720691206No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10900.11215526404333104No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10890.11205236930567661No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10780.11092052719147785No Hit
GGGCATTAGCAATTATTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTGAACTCCTGATCTATGTTGCGTC10740.11050894824086012No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10740.11050894824086012No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10740.11050894824086012No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10680.10989157981493353No Hit
TCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10640.1094800008643158No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10550.10855394822542591No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10540.10845105348777147No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10410.10711342189826385No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAT10390.10690763242295498No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10340.10639315873468283No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10070.10361500081801317No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10000.10289473765443215No Hit
GCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAAAGCGGGAGG9890.10176289554023339No Hit
CCCCTGAACTCCTGATCTATGTTGCGTCCACTTTGCAAAGTGGGGTCCCA9880.10166000080257895No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9820.10104263237665236No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9760.10042526395072576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)