FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094418
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32230.29449442534753634No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCA31110.2842606755371348No Hit
CCCAAGCACTGAGGCTTCTGTCCCATGCTAAGCAATAATAGTCAGCCTCG29910.27329594359741893No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29810.2723822159357759No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCAAGCACTGAGG27490.2511837341856585No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26040.2379346830918351No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24750.22614759625664052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24610.2248683775303403No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24210.22121346688376836No Hit
GTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC24000.2192946387943181No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22400.20467499620803023No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22000.20102008556145823No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21270.1943498736314644No Hit
CACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCC21180.1935275187359857No Hit
GTCCCATGCTAAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCACTAATGGTCAGGGAGGCTGTGTTTC20900.19096908128338533No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20780.18987260808941375No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20440.18676593403982755No Hit
GCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG20320.18566946084585598No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19740.18036984040832663No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19340.17671492976175465No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTG18970.1733341374136756No Hit
CTATTATTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAG18730.17114119102573241No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18710.1709584454934038No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATT18560.16958785400093931No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18440.16849138080696774No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18240.16666392548368172No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17790.16255215100628828No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17740.16209528717546678No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17730.16200391440930248No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAG17260.15770939439958043No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16320.1491203543801363No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15990.14610505309671443No Hit
CCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGA15960.1458309347982215No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14820.13541443945549142No Hit
ATCTACATCCAGGTCAGGAAACACAGCCTCCCTGACCATTAGTGGACTCC14420.13175952880891945No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14280.13048031008261926No Hit
GGACAGAAGCCTCAGTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14060.12847010922700466No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13930.12728226326686878No Hit
GTATAAAGGAAGATGGAACTGAGAAATACTATGTGGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGACAACG13500.12335323432180391No Hit
GGATAGGAGTTTGGGAGGGTGGCCCTGGTGCTGCTGCAGCCAAGCTGCTCCTTGGTGGCCAACATTGTTGCTGTT13440.12280499772481812No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13350.12198264282933943No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13250.12106891516769644No Hit
TTAGTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTTAGCATGG13150.12015518750605343No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13080.11951557814290335No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCAC12970.11851047771509605No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12590.1150383126008527No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACAC12480.1140332121730454No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12420.1134849755760596No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12420.1134849755760596No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12070.11028692876030913No Hit
CTTCTGTCCCATGCTAAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAG12020.10983006492948763No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11870.10845947343702314No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCAAGCACTGAGGC11850.10827672790469456No Hit
CCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCCCAAACTCCTATCCTACAGGAATAACAACCGGCC11760.10745437300921587No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11510.10517005385510837No Hit
GCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCCCAAA11370.10389083512880817No Hit
CCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGA11100.10142377044237211No Hit
AATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACT11080.10124102491004353No Hit
GTCCACTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGTAGATAAT11070.1011496521438792No Hit
ATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCACTAATGGTCAGGGAGG11040.1008755338453863No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC10980.10032729724840052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)