FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094418
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32230.29449442534753634No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29990.2740269257267333No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCA27640.25255432567812297No Hit
GTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC25450.23254368988814147No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24670.2254166141273261No Hit
CCCAAGCACTGAGGCTTCTGTCCCATGCTAAGCAATAATAGTCAGCCTCG23380.21362952729213153No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22790.2082385340884379No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22350.2042181323772087No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21450.19599458342242176No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCAAGCACTGAGG21370.1952636012931074No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21220.19389300980064292No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21040.1922483000096855No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20530.18758828893530624No Hit
GTCCCATGCTAAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCACTAATGGTCAGGGAGGCTGTGTTTC20380.18621769744284175No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19980.1825627867962698No Hit
CACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCC19490.17808552125421914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19250.17589257486627596No Hit
GCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG18300.16721216208066753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18280.16702941654833892No Hit
CTATTATTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAG17980.16428823356340996No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATT17780.16246077824012398No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTG17340.1584403765288948No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17230.1574352761010875No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16920.15460272034999425No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16820.15368899268835126No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16710.15268389226054396No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16520.15094780970342228No Hit
GTCCAAGGGCTTGAGACAGACCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAG16200.1480238811861647No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15890.14519132543507143No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15140.13833836797274898No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACAC14860.1357799305201486No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14780.1350489483908342No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14690.1342265934953555No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14500.13249051093823383No Hit
CCCAAACTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGA14370.13130266497809795No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14340.13102854667960506No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGACAGACCGCCAC13880.1268253994360473No Hit
GTATAAAGGAAGATGGAACTGAGAAATACTATGTGGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGACAACG13360.12207401559550372No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12980.11860185048126037No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12950.11832773218276747No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12900.11787086835194596No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12860.11750537728728877No Hit
GGACAGAAGCCTCAGTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12700.11604341302865998No Hit
ATCTACATCCAGGTCAGGAAACACAGCCTCCCTGACCATTAGTGGACTCC12520.1143987032377026No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12430.1135763483422239No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12320.1125712479144166No Hit
GGATAGGAGTTTGGGAGGGTGGCCCTGGTGCTGCTGCAGCCAAGCTGCTCCTTGGTGGCCAACATTGTTGCTGTT12020.10983006492948763No Hit
CTTCTGTCCCATGCTAAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAG11690.10681476364606576No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCAC11600.10599240875058706No Hit
TTAGTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTTAGCATGG11520.10526142662127266No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11440.1045304444919583No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11390.10407358066113678No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11370.10389083512880817No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11330.103525344064151No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11080.10124102491004353No Hit
GTATCAACGCAGAGTACGGGAAACAGAGCTTCAGCAAGCATAGTGGGAATCTGCACCATGCCCTGGGCTCTGCTC11010.10060141554689342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)