FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535284
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT81440.5304556030024413No Hit
GATTATTACTGCTGCTCATTTGCAGGCAACTCAGTGGTATTTGGCGGAGGGACCAAGTTGACCGTCCTACGTCAG39820.2593656939041897No Hit
ATCCTGGACTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTG39610.25799786879821585No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39330.25617410199025065No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC37630.24510123208474782No Hit
GTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAG36370.2368942814489046No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35580.23174865366928857No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33770.21995930394637084No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32750.21331558200306913No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA31800.2071278017617587No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCAAATACCAC31280.203740806261252No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29200.190192824259225No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT28110.18309316061393202No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG27980.18224641173880532No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26720.17403946110296206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26670.17371378845868257No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT26310.17136894541987022No Hit
GTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26110.17006625484275223No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGCTTATAGGTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC26030.16954517861190502No Hit
GTGTTGGTACCAGGAGACAAACCTATAAGCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGACTGA25070.16329226384173873No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24960.16257578402432382No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCAAATACCACTGAGTTGCCTGCAAATGAGCAGCAGTAATAATCAG24810.16159876609148535No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24590.16016580645665557No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGA24450.159253923052673No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24370.1587328468218258No Hit
CAGTAATACACGGCCGTGTCTGGGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCAA24170.15743015624470783No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23860.15541098585017493No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23720.15449910244619236No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23630.15391289168648928No Hit
GGGCACAGGATCCTGGACTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGC23250.15143777958996513No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG23200.1511121069456856No Hit
GAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGAT22780.14837645673373787No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22190.14453351953123983No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGCTGTTGCTTAGTTTGGAGGGTGTGGTGGTCTCCA21750.14166760026158026No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGACTCAGTCTGCCCTGACTCAGC21570.1404951787421741No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGCTGTTGCTTAGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20930.13632656889539654No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20640.13443766755857547No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20290.13215795904861902No Hit
CCACCACACCCTCCAAACTAAGCAACAGCAAGTACGCGGCCAGCAGCTAT20220.13170201734662773No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20050.13059473035607744No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19850.12929203977895945No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19820.12909663619239176No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19650.1279893492018415No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAACTCAGTGGTATTTGGCGGAGGGACCAAGTTGACCGT19230.12525369898989372No Hit
GTAATACACGGCCGTGTCTGGGGCGGTCACAGACCTCAGCTTCAGGGAGA19190.12499316087447013No Hit
CCCCTGAGGGCCGCTCATTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA18740.12206210707595468No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18590.12108508914311618No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18530.12069428196998079No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC18420.11997780215256591No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18360.11958699497943051No Hit
GCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTG18270.11900078421972743No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18150.11821916987345663No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17690.11522298154608528No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG17670.11509271248837348No Hit
GACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCT17470.11379002191125549No Hit
GTCTCTGGTTATTCCCTCAGCCGTGGTTACTCCTGGGGCTGGATCAGGCA17410.1133992147381201No Hit
GTATTACTGTTCGCGACACTGTTGGACCAGCAGCTGTGGTTTTGATGTCTGGGGCCAAGGGACGCTGGTCACCGT17160.11177085151672264No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAAC17140.11164058245901085No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17130.11157544793015495No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16970.11053329546846055No Hit
GCTTATAGGTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAATGAG16920.11020762282418106No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16790.10936087394905437No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGC16610.10818845242964818No Hit
CCTGAGGGCCGCTCATTGACATCATAAATTATGAGTTTGGGGGCTTTGCC16290.10610414750625943No Hit
GTCCCTCACCTGCGCTGTCTCTGGTTATTCCCTCAGCCGTGGTTACTCCT15810.10297769012117627No Hit
GCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTC15370.10011177085151672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8750.097.3274770
AATCGCG150.002466300368.206136
TATCGTA501.6370905E-1161.71499338
CTAACGC354.0327177E-757.420783
AATATCG601.09139364E-1051.15457536
ATCGTAT709.094947E-1249.109539
CGTATGC14500.047.95129441
TCGTATG14450.047.72851640
CCGTCTT15350.047.16687847
GTATGCC14950.046.91607342
TGCCGTC15000.046.86525345
ATGCCGT15000.046.7523644
GCCGTCT15150.046.7497446
TCTCGTA13600.046.63919438
AATCTCG13800.045.9649836
GTAATCT13700.045.8432934
TGCTTGA15600.045.75965555
ATCTCGT13950.045.56744437
ACGCGTA15050.045.51217727
CGAGACG15300.045.3365823
Produced by FastQC (version 0.11.5)