FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535284
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148190.9652285831155669No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37920.2469901334215689No Hit
GATTATTACTGCTGCTCATTTGCAGGCAACTCAGTGGTATTTGGCGGAGGGACCAAGTTGACCGTCCTACGTCAG37710.24562230831559503No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36850.24002073883398772No Hit
ATCCTGGACTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTG36800.23969506618970823No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36420.23721995409318408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC35580.23174865366928857No Hit
GTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAG34180.2226298196294627No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33010.2150090797533225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30560.19905112018362725No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA30210.19677141167367082No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCAAATACCAC30080.1959246627985441No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29210.1902579587880809No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26400.1719551561795733No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26360.1716946180641497No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT26360.1716946180641497No Hit
GTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25870.16850302615021065No Hit
GTGTTGGTACCAGGAGACAAACCTATAAGCACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGACTGA25840.16830762256364293No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGCTTATAGGTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGC25680.16726547010194856No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG25480.1659627795248306No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25390.1653765687651275No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25190.1640738781880095No Hit
GGGCACAGGATCCTGGACTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGC24840.16179416967805305No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT24790.16146849703377356No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24670.16068688268750275No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCAAATACCACTGAGTTGCCTGCAAATGAGCAGCAGTAATAATCAG23700.15436883338848056No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGA23680.15423856433076877No Hit
CAGTAATACACGGCCGTGTCTGGGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCAA23350.1520891248785241No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGACTCAGTCTGCCCTGACTCAGC22710.14792051503174655No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22000.14329596348297774No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21980.14316569442526594No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG21910.14270975272327466No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21880.14251434913670694No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGCTGTTGCTTAGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21420.1395181608093356No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAACTCAGTGGTATTTGGCGGAGGGACCAAGTTGACCGT21080.13730358682823504No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGCTGTTGCTTAGTTTGGAGGGTGTGGTGGTCTCCA20390.13280930433717802No Hit
GAGAAGGGCTTGATGCCTGGGGGAGGGAGGAGAGACCCCTCCCCTGGGAT19610.1277288110864179No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19110.12447208464362294No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19040.12401614294163166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18620.1212804927296839No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18570.12095482008540438No Hit
GTAATACACGGCCGTGTCTGGGGCGGTCACAGACCTCAGCTTCAGGGAGA18450.1201732057391336No Hit
CCCCTGAGGGCCGCTCATTGACATCATAAATTATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGGAGA18260.11893564969087153No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18090.11782836270032124No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17870.11639540306549147No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17780.11580919230578839No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17620.11476703984409399No Hit
GCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTG17340.11294327303612882No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17220.11216165868985803No Hit
CCACCACACCCTCCAAACTAAGCAACAGCAAGTACGCGGCCAGCAGCTAT17090.11131490981473136No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17070.11118464075701956No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16770.10923060489134258No Hit
GTCTCTGGTTATTCCCTCAGCCGTGGTTACTCCTGGGGCTGGATCAGGCA16700.10877466318935129No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16600.10812331790079228No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16440.10708116543909792No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16310.10623441656397123No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG16290.10610414750625943No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16200.10551793674655635No Hit
GTATTACTGTTCGCGACACTGTTGGACCAGCAGCTGTGGTTTTGATGTCTGGGGCCAAGGGACGCTGGTCACCGT16120.10499686051570914No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15970.10401984258287067No Hit
GCTTATAGGTTTGTCTCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCAATGAG15860.10330336276545576No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCGC15570.10141446142863471No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAAC15460.1006979816112198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)