FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300667
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68860.5294206741617955No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTG61390.4719886027707322No Hit
GTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGTCTGGAGGTCAGA48740.37473081119148866No Hit
CTCATATACAGCCGACAGCACTCTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGC43120.3315222112962042No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40280.30968726045944117No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCGTG39120.30076875941343945No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37870.2911583057000754No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC36260.2787800413172626No Hit
CCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACTCATATACAGCCG35880.2758584633884No Hit
GTCTGGAGGTCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCC35290.27132232923569216No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31340.24095329550146194No Hit
CTCCTGGACAGTCGATCACCATCTCGTGCACTGGAACCAGCAGTGACGTT30940.23787795031318545No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28050.2156585813278879No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27960.2149666286605257No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27030.2078164510977829No Hit
GATTATTACTGCAACTCATATACAGCCGACAGCACTCTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGT26450.20335720057478202No Hit
GTCGATCACCATCTCGTGCACTGGAACCAGCAGTGACGTTGGCCGTTATAACTATGTCTCGTGGTACCAGCAACA26250.2018195279806438No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25480.1958994884932116No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25080.19282414330493508No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24610.18921061270871023No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24590.18905684544929638No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24520.188518660041348No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24130.18552019848277845No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23540.18098406433007064No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22390.17214244691377578No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTGCAGTAATAAT22350.17183491239494814No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22150.1702972398008099No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22000.16914398535520622No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21820.1677600800204818No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTCCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGATC21790.16752942913136107No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21570.165837989277809No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21460.16499226935103298No Hit
TCTCTGACCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACTCATAT21100.16222445868158414No Hit
CTACAACCCCTCCCTCGAGAGTCGAGTCACCATATCAATTGAGACGTCCAAGAACCAGTTCTCCCTGAAGCTGAC20870.16045613519832516No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20820.16007171704979062No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20770.15968729890125605No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGGTCAGAGATGGTCAGGGAGG20370.15661195371297956No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20250.15568935015649663No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20100.15453609571089294No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20010.15384414304353075No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19750.15184516867115103No Hit
GTACCAGCAACACCCAGGCAAAGCCCCCAAACTCATTATTTCTGAGGTCA19700.15146075052261648No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19120.14700149999961556No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19050.1464633145916672No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18750.14415680570045983No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC18690.14369550392221836No Hit
ACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGG18500.14223471495778706No Hit
GTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTG18250.14031262421511426No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT18070.13892871888038985No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18040.1386980679912691No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17720.13623779184064794No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCA17690.1360071409515272No Hit
TAGTTATAACGGCCAACGTCACTGCTGGTTCCAGTGCACGAGATGGTGAT17670.13585337369211337No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17550.1349307701356304No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17080.13131723953940555No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16750.12878007975907746No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16660.12808812709171524No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16530.1270886399055254No Hit
GGTCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAG16500.12685798901640466No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16110.1238595274578351No Hit
TTATAACTATGTCTCGTGGTACCAGCAACACCCAGGCAAAGCCCCCAAAC15980.12286004027164525No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15790.1213992513072139No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15760.12116860041809319No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15740.12101483315867935No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15450.1187852078971789No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15210.11694000078421303No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15200.11686311715450612No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14970.11509479367124713No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14870.11432595737417801No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14800.11378777196622963No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14490.11140437944531535No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14430.1109430776670739No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14260.10963605596205639No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTG14260.10963605596205639No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14250.10955917233234948No Hit
GACCATCTCTGACCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACT14210.10925163781352185No Hit
GGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14150.10879033603528036No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14050.10802149973821124No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13960.10732954707084903No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13790.10602252536583154No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13700.10533057269846932No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13660.10502303817964169No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13600.10456173640140021No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13540.10410043462315874No Hit
CCTTGGGGCTGGTCGGGGATGCGGAAGAGACGGTGACCATTGTCCCTCGGCCCCAGATATCAAAAGCAAACTTCC13460.10348536558550345No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13450.10340848195579654No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13450.10340848195579654No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13380.10287029654784816No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGTTGAGTCAGGGCAGACTGGGCC13360.1027165292884343No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13280.10210146025077901No Hit
CCCCAGGGAAGGGACTGGAGTGGATTGGCTATATCTCTTACATTGGAAGC13280.10210146025077901No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13150.10110197306458917No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT13140.10102508943488225No Hit
GGGTTGTAGTGGGTGCTTCCAATGTAAGAGATATAGCCAATCCACTCCAGTCCCTTCCCTGGGGTCTGCCGGATC13080.10056378765664077No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13010.10002560224869239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)