FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300667
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62920.48375179811588975No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTG56430.43385432243610395No Hit
GTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGTCTGGAGGTCAGA45600.35058935146351833No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCGTG44740.3439773593087239No Hit
CTCATATACAGCCGACAGCACTCTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGC40710.3129932565368384No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39750.30561242808497485No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38110.2930035128130413No Hit
CCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACTCATATACAGCCG34400.264479686191777No Hit
GTCTGGAGGTCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCC33850.26025108655789686No Hit
CTCCTGGACAGTCGATCACCATCTCGTGCACTGGAACCAGCAGTGACGTT32660.2511019346227743No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC32410.2491798438801015No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31450.24179901542823798No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29480.22665294037597633No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28800.2214248535559063No Hit
GTCGATCACCATCTCGTGCACTGGAACCAGCAGTGACGTTGGCCGTTATAACTATGTCTCGTGGTACCAGCAACA26970.20735514931954144No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26130.20089692442416085No Hit
GATTATTACTGCAACTCATATACAGCCGACAGCACTCTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTGGGT25700.19759092834676362No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25520.1962070230120392No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25420.1954381867149701No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24220.18621215115014064No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23700.18221420240538125No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23440.18021522803300152No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23350.17952327536563933No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22700.17452583943469005No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22650.1741414212861555No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22470.17275751595143107No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTCCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGATC22000.16914398535520622No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21760.16729877824224032No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21440.16483850209161915No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21340.16406966579455No Hit
TCTCTGACCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACTCATAT21320.16391589853513622No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20860.16037925156861824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20850.16030236793891134No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC19980.15361349215441No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19830.15246023770880635No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19730.1516914014117372No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19680.15130698326320263No Hit
CTACAACCCCTCCCTCGAGAGTCGAGTCACCATATCAATTGAGACGTCCAAGAACCAGTTCTCCCTGAAGCTGAC19380.1490004743719953No Hit
GTACCAGCAACACCCAGGCAAAGCCCCCAAACTCATTATTTCTGAGGTCA19240.14792410355609853No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19230.1478472199263916No Hit
GACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTGCAGTAATAAT19120.14700149999961556No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGGTCAGAGATGGTCAGGGAGG18990.14600201281342573No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18940.14561759466489116No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18630.1432342021439769No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18520.14238848221720088No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18410.14154276229042484No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18020.13854430073185528No Hit
GTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGGCTGTATATGAGTTG17980.1382367662130276No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17510.13462323561680278No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17390.13370063206031982No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCA17370.133546864800906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17210.1323167267255954No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17130.13170165768794012No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT16980.13054840324233644No Hit
TTATAACTATGTCTCGTGGTACCAGCAACACCCAGGCAAAGCCCCCAAAC16300.1253203164222664No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16230.12478213101431804No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15850.12186055308545539No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15790.1213992513072139No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15770.1212454840478001No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15750.12109171678838626No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCT15680.12055353138043787No Hit
TAGTTATAACGGCCAACGTCACTGCTGGTTCCAGTGCACGAGATGGTGAT15680.12055353138043787No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15530.1194002769348342No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15470.11893897515659273No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15360.1180932552298167No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15320.11778572071098904No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15140.11640181537626465No Hit
GACCATCTCTGACCTCCAGACTGAGGACGAGGCTGATTATTACTGCAACT15100.11609428085743699No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14940.11486414278212641No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14700.1130189356691605No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT14660.11271140115033287No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14500.11148126307502229No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14470.11125061218590154No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC14410.11078931040766006No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14400.11071242677795315No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14390.11063554314824625No Hit
GGTCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAG14280.1097898232214702No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14220.10932852144322876No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTG14060.10809838336791815No Hit
ACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAAAACCAGAGTGCTGTCGG14060.10809838336791815No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14040.10794461610850432No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCGTGCA13650.10494615454993478No Hit
GGGTTGTAGTGGGTGCTTCCAATGTAAGAGATATAGCCAATCCACTCCAGTCCCTTCCCTGGGGTCTGCCGGATC13650.10494615454993478No Hit
CTCGTGCACTGGAACCAGCAGTGACGTTGGCCGTTATAACTATGTCTCGTGGTACCAGCAACACCCAGGCAAAGC13540.10410043462315874No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13470.10356224921521034No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13370.10279341291814124No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13300.10225522751019286No Hit
GGACCAAACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG13280.10210146025077901No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13210.10156327484283063No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13160.10117885669429609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)