FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517247
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG71590.4718414338601427No Hit
GTATAAAACTTATTCGGGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT64290.423727975734999No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63130.41608254951237333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56540.37264861950625044No Hit
AAGTATAGGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGACGT50140.3304669575883162No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48820.32176698981774227No Hit
GTTTTATACTGCTGACAGTAGTAAGTTGCAAAATCATCAGGGTGCAGGCTGGTGATGGTGAGGGTGAAGTCTGTC48350.318669274020644No Hit
GTATAGGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT44580.2938216387971108No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39770.26211948351191333No Hit
CCTATACTTTGAGTGGCCCGGCAAGTGATAGTGATTCTGTCTCCGACATA38970.2568467757721716No Hit
ATTCTAGAGGGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACCCTCACCATCACCAGC38830.2559240519177168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38470.25355133343483294No Hit
AGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC38270.2522331564998975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36080.23779911906235437No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35670.23509685634573674No Hit
CCTTCCACCCTGTCTGCATATGTCGGAGACAGAATCACTATCACTTGCCG35200.2319991405486384No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34520.2275173389698579No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33940.22369462585854513No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33470.2205969100614468No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33310.21954236851349845No Hit
GACTTCACCCTCACCATCACCAGCCTGCACCCTGATGATTTTGCAACTTA33060.21789464734482916No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30420.20049471180368128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30070.19818790216754426No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29590.1950242775236992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28600.18849930169576873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28450.18751066899456714No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC27940.18414931781048174No Hit
CACCTATACTTTGAGTGGCCCGGCAAGTGATAGTGATTCTGTCTCCGACATATGCAGACAGGGTGGAAGGAGA27140.17887661007073996No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC26980.17782206852279162No Hit
CACCATCACCAGCCTGCACCCTGATGATTTTGCAACTTACTACTGTCAGCAGTATAAAACTTATTCGGGGACGTT26830.17683343582159003No Hit
GTCTGCATATGTCGGAGACAGAATCACTATCACTTGCCGGGCCACTCAAA25010.16483802571367745No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24610.16220167184380657No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23580.15541306062888904No Hit
CTCTAGAATAGACGTCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT23180.15277670675901814No Hit
ACTTACTACTGTCAGCAGTATAAAACTTATTCGGGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA23060.15198580059805686No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACTTCCAGATGACCCAGTCTCCTTCCACC22790.15020626173589402No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22410.14770172555951666No Hit
GACCCAGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGG21690.1429562885937491No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21420.14117674973158623No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20900.13774948970075407No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20690.13636540391907184No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20560.1355085889113638No Hit
TCACCAGCCTGCACCCTGATGATTTTGCAACTTACTACTGTCAGCAGTAT19820.13063133425210266No Hit
CAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACTT19480.1283904334627124No Hit
GGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19130.12608362382657537No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19080.12575407959284152No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18770.12371090534369156No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18310.12067909839334004No Hit
GTCTCCTTCCACCCTGTCTGCATATGTCGGAGACAGAATCACTATCACTT17940.11824047106370947No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17750.1169882029755208No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17560.11573593488733212No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17340.11428594025890314No Hit
GTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17330.11422003141215635No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17210.1134291252511951No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17200.11336321640444831No Hit
GTAGTAAGTTGCAAAATCATCAGGGTGCAGGCTGGTGATGGTGAGGGTGAAGTCTGTCCCAGATCCACTGCCG16810.1107927713813242No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16780.11059504484108389No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16700.11006777406710971No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16690.11000186522036294No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16380.10795869097121298No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16230.10697005827001141No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16070.10591551672206306No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15810.10420188670664697No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15760.1038723424729131No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15760.1038723424729131No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15750.10380643362616634No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15730.10367461593267278No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15500.10215871245749704No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15400.10149962399002932No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15240.10044508244208095No Hit
CTAGAATAGACGTCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACTCAC15180.10004962936160033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG405.205176E-542.8360935
GTATCAA38900.041.0579031
GTGGTAT3100.040.5591281
TTAACGG702.323759E-839.16442535
GACCGAT2150.037.99347326
CTCGGTA451.08151E-437.83756627
TATCAAC42750.037.1218262
ACGAGAC2200.037.07979622
ACCGATC2250.036.3240627
AGACCGA2250.036.289325
CGTATGC1250.035.95152341
CGATCAG2300.035.58024229
ATCAACG44750.035.5386853
TCAACGC45350.034.8436974
CAACGCA45550.034.765315
ATGCGGT400.002533935234.67223743
TAACGGC807.447852E-834.3110736
AACGCAG46400.033.9853326
GCATCGA400.00279607733.983098
ATGCCGT1450.033.5025644
Produced by FastQC (version 0.11.5)