FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517247
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAACTTATTCGGGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT60890.4013189678410964No Hit
GCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG60810.40079169706712225No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59610.39288263545750957No Hit
GTTTTATACTGCTGACAGTAGTAAGTTGCAAAATCATCAGGGTGCAGGCTGGTGATGGTGAGGGTGAAGTCTGTC50300.33152149913626455No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43970.28980119914555774No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43760.2884171133638755No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43220.2848580356395498No Hit
AAGTATAGGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGACGT41180.2714126309032082No Hit
GTATAGGTGAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT37500.24715817530039605No Hit
ATTCTAGAGGGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACCCTCACCATCACCAGC35880.23648094212741894No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35590.23456958557176252No Hit
CCTATACTTTGAGTGGCCCGGCAAGTGATAGTGATTCTGTCTCCGACATA35500.2339764059510416No Hit
AGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC34270.2258696178011886No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34200.2254082558739612No Hit
GACTTCACCCTCACCATCACCAGCCTGCACCCTGATGATTTTGCAACTTA34160.22514462048697412No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31960.2106446742026842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31720.20906286188076167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30430.20056062065042804No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30070.19818790216754426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28410.18724703360758005No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27380.1804584223926625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27190.1792061543044738No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27090.1785470658370061No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26570.17511980580617395No Hit
CCTTCCACCCTGTCTGCATATGTCGGAGACAGAATCACTATCACTTGCCG26380.17386753771798527No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25180.1659584761083726No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24880.16398121070596944No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC24860.1638493930124759No Hit
CACCTATACTTTGAGTGGCCCGGCAAGTGATAGTGATTCTGTCTCCGACATATGCAGACAGGGTGGAAGGAGA24550.16180621876332593No Hit
CACCATCACCAGCCTGCACCCTGATGATTTTGCAACTTACTACTGTCAGCAGTATAAAACTTATTCGGGGACGTT24000.15818123219225347No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23880.15739032603129222No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC23820.15699487295081158No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23460.15462215446792776No Hit
GGGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA23390.15416079254070036No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23320.15369943061347296No Hit
ACTTACTACTGTCAGCAGTATAAAACTTATTCGGGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA22410.14770172555951666No Hit
GTCTGCATATGTCGGAGACAGAATCACTATCACTTGCCGGGCCACTCAAA21700.14302219744049582No Hit
CTCTAGAATAGACGTCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT20960.1381449427812347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20700.1364313127658186No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20280.13366314120245418No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19880.13102678733258327No Hit
GACCCAGTCAGGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGG19130.12608362382657537No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACTTCCAGATGACCCAGTCTCCTTCCACC19050.12555635305260118No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18700.12324954341646416No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17950.11830637991045626No Hit
GGACACAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17880.11784501798322883No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17690.11659274989504016No Hit
GTAGTAAGTTGCAAAATCATCAGGGTGCAGGCTGGTGATGGTGAGGGTGAAGTCTGTCCCAGATCCACTGCCG17540.11560411719383859No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17250.11369276063818218No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17100.1127041279369806No Hit
TCACCAGCCTGCACCCTGATGATTTTGCAACTTACTACTGTCAGCAGTAT17080.11257231024348706No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17000.11204503946951289No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16760.11046322714759033No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16570.10921095905940166No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16540.10901323251916134No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16290.10736551135049205No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16240.1070359671167582No Hit
CAGCATAGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACTT16150.10644278749603724No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACCCTCTAGAAT15790.10407006901315342No Hit
GGGTGGAAGGAGACTGGGTCATCTGGAAGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG15760.1038723424729131No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15380.10136780629653576No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15350.10117007975629544No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15280.10070871782906804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTT200.00786982450.87975314
GTAACGG350.001122029340.92718561
GTATCAA37000.040.7508241
GTGGTAT3900.039.1834831
TATCAAC41250.036.189512
ATCAACG42750.035.0714873
TCAACGC43200.034.6207924
AACGCAG44000.034.1466036
CAACGCA44050.034.0308535
ACCGATG502.058344E-433.915368
ACGCAGA46100.032.5911187
CGCAGAG46500.032.5295758
TAGTACC751.9084855E-631.6532924
AGAGTAC47900.031.64440211
CAGAGTA48700.030.989410
GCAGAGT49300.030.6132589
GGCGATA450.00498260430.20074825
CGGGATT1052.735942E-829.08948917
TTAACGG854.6695022E-628.2196135
AATGGTA7050.027.88768670
Produced by FastQC (version 0.11.5)