FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563822
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29690.5265846313198137No Hit
GCCTTGGGCTGACCCAGGACGGCCAGGTTGGTCCCTCCGCCGAAAATGACAGTGCTTCTGCTTGTATATGAGCTG25240.44765901295089583No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17680.31357414219381297No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16280.2887436105721309No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15670.27792459322268376No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGGTTGGTCCCTC15560.2759736228809802No Hit
GTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14070.24954684279790432No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12810.22719936433839047No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACATT11980.21247840630553616No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11590.20556132963949614No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11310.20059522331515975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11250.19953105767423052No Hit
CATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11080.1965159216915977No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10910.1935007857089649No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10820.19190453724757106No Hit
GTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACATTGGGACTTACGACTATGTCTCCTGGTACCAACAACA10550.1871157918633895No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10390.18427801682091158No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10150.18002135425719465No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTCGTAA10020.1777156620351813No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9970.17682885733440695No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9930.17611941357378746No Hit
CATTGGGACTTACGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGCGGT9920.17594205263363258No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9820.17416844323208389No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTTTTACTGCAGCTCATAT9550.16937969784790236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9410.16689664468573415No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9400.16671928374557926No Hit
CTCCTGCACCGGAACCAGCAGTGACATTGGGACTTACGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGT9290.16476831340387568No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9080.16104373366062338No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8840.15678707109690648No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8650.1534172132339639No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG8650.1534172132339639No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8620.1528851304134993No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8310.14738694126869828No Hit
GGTTGGTCCCTCCGCCGAAAATGACAGTGCTTCTGCTTGTATATGAGCTGCAGTAAAAATCAGCCTCGTCGTCAG8290.14703221938838854No Hit
GTCCCCAAACTCATCATTTATGCGGTCACTAATCGGCCCTCAGGTGTTTCTCATCGCTTCTCTGGCTCCAAGT8250.14632277562776905No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8110.14383972246560087No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGC8040.14259819588451675No Hit
AGTCTGTCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7960.14117930836327777No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC7920.14046986460265828No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7720.13692264579956084No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGG7550.13390750981692803No Hit
ATATTATACAGAGCCTGTGAAGGGCCGATTCACCATTTCTAGAGACAATG7450.1321339004153793No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7420.1316018175949147No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7270.12894140349259164No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG7260.12876404255243676No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGGTTGGTCCCTCCGCCGAAAATGACAGTGCTT7260.12876404255243676No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7180.12734515503119778No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7080.12557154562964906No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6690.11865446896360907No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6630.11759030332267985No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6620.11741294238252498No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6620.11741294238252498No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6600.11705822050221523No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT6510.1154619720408214No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6470.11475252828020191No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6410.11368836263927268No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6340.11244683605818857No Hit
GTATATGAGCTGCAGTAAAAATCAGCCTCGTCGTCAGCCTGGAGCCCAGA6290.11156003135741421No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6220.11031850477633011No Hit
CCTGAGGGCCGATTAGTGACCGCATAAATGATGAGTTTGGGGACTTTGCC6190.10978642195586549No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGT5970.10588448127245834No Hit
GACCCAGGACGGCCAGGTTGGTCCCTCCGCCGAAAATGACAGTGCTTCTGCTTGTATATGAGCTGCAGTAAAAAT5940.10535239845199372No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5810.10304670622998037No Hit
GACCATCTCTGGGCTCCAGGCTGACGACGAGGCTGATTTTTACTGCAGCT5740.10180517964889628No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCTGCCT5670.10056365306781218No Hit
GATTTTTACTGCAGCTCATATACAAGCAGAAGCACTGTCATTTTCGGCGGAGGGACCAACCTGGCCGTCCTGGGT5650.10020893118750243No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5640.10003157024734757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1300.066.1594970
GGTATCA4150.047.630661
GTATCAA9550.034.615861
GGATGGG3300.034.0821670
CGAGACG1800.034.07753823
TATACAC1950.033.2037543
AGACGTA1750.033.1127125
CTTATAC1900.032.2868461
CGTAGAG1800.032.2243628
ACGTAGA1800.032.2186427
TCGTATG1400.032.0126340
TGCCGTC1650.031.57054545
CACGAGA1950.031.4506121
CGTATGC1550.031.18922241
AGGTATC553.4987665E-430.979582
ACGAGAC2000.030.66706322
GACGTAG1900.030.50666626
TATCAAC10950.029.87622
GTATGCC1750.029.64323642
TACACAT2100.029.2093185
Produced by FastQC (version 0.11.5)