FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734832
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33620.45751954188168176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31960.434929344394365No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28180.38348901517625794No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25140.3421190149585211No Hit
GTCCTGATCTATGCTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTT24510.3335456267555033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24140.3285104622553182No Hit
GATCAGGACCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGCTAAAT23230.31612667929540356No Hit
GATCTATGCTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA22100.30074901474078425No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21810.29680253445685545No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21280.2895900015241579No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21140.2876848041457095No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20490.2788392448886276No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19720.26836065930716135No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19590.266591547455745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19540.26591111982058485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19290.26250898164478415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18850.25652121845537484No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACAC18800.25584079082021466No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18700.2544799355498944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18530.25216648159034993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17880.243320922333268No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT17410.23692490256276263No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17380.23651664598166658No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16470.22413286302175192No Hit
GCATAGATCAGGACCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGC16350.22249983669736756No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC16090.21896161299453482No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15780.21474296165654191No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG15730.21406253402138178No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15390.20943562610229277No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15160.20630565898055608No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC14680.19977355368301872No Hit
GAATTCACTCTCACAATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATGAATAC14590.19854878393973044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14540.1978683563045703No Hit
GTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTGCAACCA13620.18534848781762361No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13190.17949681015524638No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13170.1792246391011823No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12830.17459773118209332No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12810.17432556012802927No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12570.17105950747926055No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12390.16860996799268405No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGTTGCAGCATAGATCAGGACCTTAGGGGCTTTTCCTGGGTGTTG12170.1656160863979794No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12020.16357480349249895No Hit
ACTTATTACTGTCAACAGCTTAATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAA12000.1633026324384349No Hit
GGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTGCAAC11780.16030875084373028No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11520.1567705271408975No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAATTGGATGTCACATCTGGCACCTGGGAGCCAGAG11470.15609009950573735No Hit
CCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGCTAAATAACCTCTA11410.1552735863435452No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11020.14996625078929607No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10990.14955799420819998No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10780.14670019814052734No Hit
GTCAGGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTG10740.14615585603239925No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10620.1445228297080149No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10470.14248154680253447No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10170.13839898099157358No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10160.13826289546454157No Hit
CCTCTAAGACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGA10150.13812680993750953No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9860.13418032965358068No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9750.13268338885622835No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9590.1305060204237159No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9520.1295534217344917No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9470.12887299409933156No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9440.12846473751823548No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAACAACCTGCAGCCTGAAGATTTTG9440.12846473751823548No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9430.1283286519912034No Hit
GCTTAATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT9410.12805648093713937No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9230.12560694145056284No Hit
ATCTCCACCTTGGTCCCCCCGCCGAAAGTGAGAGGGTATTCATTAAGCTG9220.12547085592353083No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9170.12479042828837067No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT9110.12397391512617849No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9090.12370174407211444No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9080.12356565854508242No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9010.1226130598558582No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8980.12220480327476212No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8810.11989134931521762No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8790.11961917826115358No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8740.11893875062599342No Hit
GTGAGAGGGTATTCATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTC8670.11798615193676923No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8650.11771398088270515No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8640.11757789535567313No Hit
CAATTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT8620.11730572430160907No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG8550.11635312561238487No Hit
CTAAGACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8540.11621704008535284No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8430.11472009928800052No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8410.11444792823393647No Hit
GGACCCCACTTTGCAAAGTGGTTGCAGCATAGATCAGGACCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGG8390.1141757571798724No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG8240.11213447427439198No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8230.11199838874735993No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8120.11050144795000762No Hit
TCATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTC8090.11009319136891153No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTC8090.11009319136891153No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8010.1090045071526553No Hit
CTACCTGGACTGGGTCCGCCAGGCTCCTGGGAAGGGGCTGGAGTGGGTTG7840.1066910531931108No Hit
ATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCT7660.10424151370653428No Hit
ATGCTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA7660.10424151370653428No Hit
ATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGA7610.10356108607137414No Hit
GGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTGCAACCACTTTGCAAAGTGGGGTCCCAT7590.10328891501731009No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC7590.10328891501731009No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7550.10274457290918196No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7540.10260848738214993No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA7510.10220023080105385No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7490.10192805974698978No Hit
GGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC7460.10151980316589371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTG150.00199954671.9073963
AATACCT150.002487417368.0560765
TGACGGA200.00719274852.04802345
GGTATCA6050.047.2487561
TAATACC306.7530817E-445.370724
AACGCTG350.001414130339.04329731
GACAGGT1500.038.5677341
GTATCAA15600.038.1750761
ATGCCGT1009.094947E-1238.1394444
GTGGTAT1450.037.5507351
TGGTATC1450.035.2038152
CGTATGC1003.765308E-1034.5357141
TCGTATG1003.8198777E-1034.4856940
TATCAAC17650.033.355532
ATCAACG17950.032.9853973
CGTACTA1057.203198E-1032.5049929
ACCGTAC1057.2759576E-1032.47838627
TCAACGC18350.032.266374
CAACGCA18500.032.004755
TAGGTAG450.00410192531.42332856
Produced by FastQC (version 0.11.5)