FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734832
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31650.43071069305637205No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25270.3438881268099375No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24910.33898904783678446No Hit
GTCCTGATCTATGCTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTT24160.3287826333093823No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23720.322794870119973No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22960.3124523700655388No Hit
GATCAGGACCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGCTAAAT20410.27775056067237136No Hit
GATCTATGCTGCAACCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA19330.26305332375291224No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19000.2585625013608553No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18990.25842641583382325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.25434385002286236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18300.24903651446861325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18260.24849217236048513No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18070.24590654734687659No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18030.24536220523874844No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17690.24073529731965948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17300.2354279617654103No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17200.23406710649509005No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACAC16730.22767108672458466No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16030.21814509983234262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15980.2174646721971825No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15670.2132460208591896No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14980.20385611949397955No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT14870.20235917869662726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14230.1936497049665774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14090.19174450758812897No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14070.19147233653406492No Hit
GTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTGCAACCA13960.1899753957367126No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC13850.1884784549393603No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCCCCGCCGAAAGTG13850.1884784549393603No Hit
GCATAGATCAGGACCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGC13720.1867093430879439No Hit
GAATTCACTCTCACAATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATGAATAC13240.1801772377904065No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13240.1801772377904065No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13130.1786802969930542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12840.17473381670912536No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG12670.17242036274958084No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC12530.17051516537113245No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.16997082326300433No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11940.16248611927624274No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11590.1577231258301217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11550.1571787837219936No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAATTGGATGTCACATCTGGCACCTGGGAGCCAGAG11310.1539127310732249No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11310.1539127310732249No Hit
ACTTATTACTGTCAACAGCTTAATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAA11220.15268796132993664No Hit
GGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTGCAAC11070.1506466784244562No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11030.15010233631632808No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGTTGCAGCATAGATCAGGACCTTAGGGGCTTTTCCTGGGTGTTG10980.14942190868116795No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10910.14846930999194374No Hit
CCTTAGGGGCTTTTCCTGGGTGTTGCTGAAACCAGGCTAAATAACCTCTA10620.1445228297080149No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10590.1441145731269188No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10240.13935157968079778No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10150.13812680993750953No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10050.13676595466718924No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9800.1333638164913885No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9730.1324112178021643No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9630.13105036253184402No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9610.13077819147777997No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT9460.12873690857229952No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9280.126287369085723No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9270.12615128355869096No Hit
CCTCTAAGACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGA9210.1253347703964988No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9070.12342957301805038No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9040.12302131643695428No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8800.1197552637881856No Hit
GTGAGAGGGTATTCATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTC8630.11744180982864111No Hit
GCTTAATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT8610.11716963877457705No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8570.11662529666644894No Hit
GTCAGGGTCTTAGAGGTTATTTAGCCTGGTTTCAGCAACACCCAGGAAAAGCCCCTAAGGTCCTGATCTATGCTG8490.11553661245019269No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8400.11431184270690445No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8380.11403967165284037No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8370.11390358612580835No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAACAACCTGCAGCCTGAAGATTTTG8330.11335924401768022No Hit
ATCTCCACCTTGGTCCCCCCGCCGAAAGTGAGAGGGTATTCATTAAGCTG8200.11159013216626384No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC8200.11159013216626384No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8090.11009319136891153No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTC8040.10941276373375138No Hit
CTAAGACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8020.10914059267968733No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA7970.10846016504452718No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7930.10791582293639906No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7870.1070993097742069No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7780.10587454003091862No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7770.1057384545038866No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7720.10505802686872646No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG7720.10505802686872646No Hit
ATCAACAACCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCT7710.10492194134169443No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGT7710.10492194134169443No Hit
TCATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTC7670.1043775992335663No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7620.10369717159840616No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGTTGTTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG7610.10356108607137414No Hit
ATGAATACCCTCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAGCGA7450.10138371763886167No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7440.10124763211182965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAT200.006206674554.0249363
AACGGAC200.006276158653.87310862
TGCGAGT200.0078460850.9160315
GTGGTAT2050.049.6910931
CTCTAAT405.492232E-542.444481
TGGTATC2250.040.727282
TTTAAGT1452.413799E-838.9791770
GTATCAA14100.037.80871
GGTATCA5150.036.9225771
GACAGGT1151.8189894E-1235.431911
TAACGGC400.002678349134.28019336
GTGCTAG601.49628795E-533.955581
TATCAAC15950.033.1946452
GATTCAC1450.032.7847021
ATCAACG16350.032.5790333
TCAACGC16750.031.7923684
CACTCGT1355.456968E-1231.59244358
CAACGCA17050.031.4319115
CGGTACC450.00445594230.89626545
TCTAATA553.58412E-430.8542
Produced by FastQC (version 0.11.5)