FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553837
Sequences flagged as poor quality0
Sequence length21-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC129740.8349653148946768No Hit
GTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC126570.8145642046109083No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71600.460794793791112No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA65750.42314605714756437No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG61900.3983686834590759No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTACCC58240.3748140892513179No Hit
GATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG55580.3576951765210894No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGCCTTGAGCTCTGG55310.35595754252215644No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG54630.3515812791174364No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54410.35016542919237986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45510.2928878640423674No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44680.2875462484160179No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC43140.27763529894062244No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT42850.2757689513121389No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40780.26244709065365285No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40690.26186787932067523No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG39000.2509915776236504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37800.24326875985061497No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36450.23458058985595015No Hit
CCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAAACACTCGCTCT36060.23207067407971363No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36040.23194196045016308No Hit
CTCATCTACAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG35550.2287884765261736No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT35450.22814490837842066No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35210.22660034482381358No Hit
CCCCAGAAGAGCGAGTGTTTGTGTTGTAGATGAGCGTGCGTGGAGTCTGG35160.2262785607499371No Hit
CACTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGA35060.22563499260218417No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC34150.2197785224576323No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33960.21855574297690172No Hit
CTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAAACA33130.21321412735055223No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT32970.2121844183141475No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG32890.21166956379594515No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31980.20581309365139328No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31640.20362496194903326No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA31590.20330317787515678No Hit
GTAATACACCGCCGAGTCCTCGGCTCTCAGACTGTTCATTTCCAGATACA31530.20291703698650504No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30680.19744670773060494No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTACCCA28550.18373870618346713No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28050.18052086544470236No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGCCTTGAGCTCTGGCTCAGTC27850.17923372914919647No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27620.17775352240936468No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27180.1749218225592517No Hit
GTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC27170.17485746574447641No Hit
GTAGATGAGCGTGCGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGG27160.17479310892970112No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA26190.1685504978964975No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25870.16649107982368808No Hit
CTCCACGCACGCTCATCTACAACACAAACACTCGCTCTTCTGGGGTCCCT25540.16436730493610333No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25100.16153560508599035No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT24940.16050589604958565No Hit
ACCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT24780.15947618701318092No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG23850.1534910032390785No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG23730.15271872146177495No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23520.15136722835149377No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT23320.15008009205598785No Hit
CATCTACAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC23090.1485998853161561No Hit
GGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTAACTAGAAGAGACTGAG23080.14853552850138077No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC22810.14679789450244782No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22700.14608996953991957No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22620.14557511502171722No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTT22190.14280777198637953No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22180.14274341517160422No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21900.14094142435789597No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21850.1406196402840195No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21740.13991171532149124No Hit
CTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTC21660.1393968608032889No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21550.13868893584076064No Hit
AACTAGAAGAGACTGAGCCAGAGCTCAAGGCACAAGTGAGTGTGACTGTC21180.13630773369407473No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC21140.13605030643497354No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20780.13373346110306294No Hit
GTCACACTCACTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTA20760.13360474747351236No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20520.13206018391890528No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC20300.13064433399384878No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC20110.12942155451311818No Hit
TGCGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTAACTAGAAGAGACTGAGCCAGAGCTCAAGG19970.12852055910626403No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC19350.12453043659019576No Hit
GTGTATTACTGTGCAAGAGGGGAATGGCGCTATAGCACCAGTATCGTCGA19150.12324330029468986No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18950.12195616399918395No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18700.12034724362980159No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18650.1200254595559251No Hit
GTACCAGCAGACCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAA18340.11803039829789096No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17950.11552048252165446No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17890.11513434163300268No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC17790.11449077348524973No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA17640.11352542126362032No Hit
CCCAACCACTACCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGG17610.11333235081929442No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17290.11127293274648499No Hit
GTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT17260.1110798623021591No Hit
TTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC16770.10792637837816965No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16680.107347167045192No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16480.10606003074968609No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16360.10528774897238255No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGAC16150.10393625586210137No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16080.10348575815867431No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTAC15920.10245604912226959No Hit
CCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTG15840.10194119460406723No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15830.10187683778929192No Hit
GGCCAGACTCCACGCACGCTCATCTACAACACAAACACTCGCTCTTCTGG15750.10136198327108956No Hit
GAGTCTGGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTAACTAGAAGAG15750.10136198327108956No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15590.10033227423468485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)