FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553837
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC118730.764108461827077No Hit
CCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC107330.6907416929832408No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68520.4409728948403211No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGCCTTGAGCTCTGG64600.41574502344840547No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA60570.3898092270939616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60430.3889082316871075No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53190.3423138977897939No Hit
GATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG52660.33890298660670326No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTACCC51660.3324673051291738No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG50330.3239078487640596No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44750.287996746119445No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC42450.2731946787211271No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41650.2680461335391035No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT41440.26669464042882235No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG39870.25659062050910103No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37990.24449153933134557No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36980.2379915010390408No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36700.23618951022533252No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36610.23561029889235485No Hit
CTCATCTACAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG35890.2309766082285336No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT35220.22666470163858884No Hit
CCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAAACACTCGCTCT34960.22499142445443118No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34890.22454092675100412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34840.22421914267712764No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG34780.2238330017884759No Hit
CTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAAACA33610.2163032544597664No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33320.21443690683128283No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33200.21366462505397926No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33110.21308541372100162No Hit
CCCCAGAAGAGCGAGTGTTTGTGTTGTAGATGAGCGTGCGTGGAGTCTGG32780.2109616388334169No Hit
CACTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGA32630.20999628661178749No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32190.20716458676167449No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30760.1979615622488073No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29550.19017438766099662No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC29380.1890803218098166No Hit
GTAATACACCGCCGAGTCCTCGGCTCTCAGACTGTTCATTTCCAGATACA28940.18624862195970363No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28670.18451098796077064No Hit
GTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC27640.1778822360389153No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA27210.1751148930035776No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGCCTTGAGCTCTGGCTCAGTC26950.17344161581941991No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT26180.16848614108172222No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26170.16842178426694693No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25660.16513958671340687No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA25470.16391680723267626No Hit
GTAGATGAGCGTGCGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGG24640.15857519160632677No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG24490.15760983938469736No Hit
CTCCACGCACGCTCATCTACAACACAAACACTCGCTCTTCTGGGGTCCCT24110.15516428042323616No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTACCCA23930.15400585775728085No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23730.15271872146177495No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23400.15059494657419023No Hit
GGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTAACTAGAAGAGACTGAG23360.15033751931508904No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23200.1493078102786843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23090.1485998853161561No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22650.1457681854660431No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT22360.14390183783755955No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG21960.14132756524654774No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21950.14126320843177245No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21900.14094142435789597No Hit
CATCTACAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC21890.14087706754312068No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21810.1403622130249183No Hit
ACCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT21310.13714437228615356No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC21200.1364364473236253No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTT21050.1354710951019959No Hit
GTCACACTCACTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTA21040.1354067382872206No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20820.1339908883621641No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20760.13360474747351236No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20700.1332186065848606No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20670.1330255361405347No Hit
CTTGTGCCTTGAGCTCTGGCTCAGTCTCTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGACTC20650.1328968225109841No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20520.13206018391890528No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19970.12852055910626403No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19560.12588192970047696No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA19430.1250452911083981No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC19260.12395122525721809No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC18580.11957496185249804No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC18300.11777297103878978No Hit
GTGTATTACTGTGCAAGAGGGGAATGGCGCTATAGCACCAGTATCGTCGA18300.11777297103878978No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC17790.11449077348524973No Hit
TGCGTGGAGTCTGGCCTGGGGTCTGCTGGTACCAACTGGGGTAGTAACTAGAAGAGACTGAGCCAGAGCTCAAGG17770.11436205985569914No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC17470.11243135541244031No Hit
AACTAGAAGAGACTGAGCCAGAGCTCAAGGCACAAGTGAGTGTGACTGTC17350.11165907363513677No Hit
GTACCAGCAGACCCCAGGCCAGACTCCACGCACGCTCATCTACAACACAA17230.11088679185783322No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGAC17050.10972836919187791No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17030.10959965556232731No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16670.10728281023041669No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16650.1071540966008661No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16570.10663924208266376No Hit
GTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT16530.10638181482356258No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT16520.10631745800878728No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16520.10631745800878728No Hit
GGCCAGACTCCACGCACGCTCATCTACAACACAAACACTCGCTCTTCTGG16490.10612438756446141No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16280.1047728944541802No Hit
GTCTCTTCTAGTTACTACCCCAGTTGGTACCAGCAGACCCCAGGCCAGAC16230.10445111038030373No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTAC15930.10252040593704487No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC15910.10239169230749429No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15860.10206990823361782No Hit
TTATTACTGTGTGCTGTATATGGGTAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC15860.10206990823361782No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC15750.10136198327108956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT252.7556368E-454.41518814
TCGCGTC200.007138679752.15023846
TTAGAGC200.00782892250.9469263
TACGTAT702.2608614E-549.32728670
GTGGTAT3000.046.436241
GGTATCA12000.043.0384671
TCTGGAG9850.038.56041770
TGGTATC3600.037.743322
GTATCAA28200.037.1104351
AATGGTA6000.036.8310470
CCCCTCG53750.034.047370
TATCAAC31850.032.315872
ATCAACG33450.030.6646163
GACAGGT1900.030.4011361
TCAACGC34100.030.081074
CAACGCA34250.029.8530395
AACGCAG34800.029.388796
GACCGGA604.821072E-429.33374853
AAGGGTT950.005995257329.07713770
TCGGTTT1205.393843E-428.77425270
Produced by FastQC (version 0.11.5)