FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459198
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTACACTTTTGGCC22950.4997844067265101No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18940.4124582424139478No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18100.39416547981480754No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17540.3819703047487141No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15100.32883418481787813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12640.275262522920396No Hit
GTGTTAGCACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT12400.27003601932064164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12240.26655168358747205No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12150.2645917447375642No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10810.23541043297226902No Hit
GTATGGTAGCTCACCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGG10480.22822399052260678No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATTGCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10360.22561073872272966No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10020.2182065252897443No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9910.21581104447319024No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTACTGGTGCTAACACTCTGA9900.21559327348986712No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9200.20034930465725026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9160.19947822072395785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9090.19795382384069615No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGCAATGCCAGTGGCCCTGCT9070.19751828187404996No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9030.19664719794075758No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8900.1938161751575573No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8870.19316286220758802No Hit
GTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATT8680.18902521352444915No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8660.18858967155780296No Hit
GCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT8640.18815412959115677No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGCAATGCCA8290.1805321451748483No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8100.17639449649170946No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC8080.17595895452506327No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGA7930.1726923897752168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7930.1726923897752168No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7930.1726923897752168No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7900.1720390768252475No Hit
GGCCAAAAGTGTACATGGGAGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT7760.16899028305872413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7570.16485263437558526No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGC7340.159843901759154No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAGTAACTTAGCCTGGTACC7280.15853727585921543No Hit
GTCAGAGTGTTAGCACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGC7270.15831950487589233No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGGTGAGCTACCATA7100.15461739815939965No Hit
GTAGTAAACGGCCGTGTCCTCAGATCTCAGGCCGCTCAGCTCCATATAGG7020.15287523029281486No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT6910.15047974947626078No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC6720.1463421007931219No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6710.14612432980979884No Hit
GACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA6700.14590655882647574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6570.14307553604327544No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6460.14068005522672136No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6420.13980897129342898No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTC6230.13567132261029013No Hit
GCCTATATGGAGCTGAGCGGCCTGAGATCTGAGGACACGGCCGTTTACTA6070.13218698687712055No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6070.13218698687712055No Hit
ATATGGAGCTGAGCGGCCTGAGATCTGAGGACACGGCCGTTTACTACTGT5850.1273960252440124No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5810.12652494131071998No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5810.12652494131071998No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5790.1260893993440738No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5760.1254360863941045No Hit
GTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA5730.12478277344413521No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5560.12108066672764253No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5530.12042735377767325No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5530.12042735377767325No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGAGACTCTGCCC5420.11803187296111917No Hit
GTACATGGGAGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAAT5420.11803187296111917No Hit
GTAGTAAAGAGGTAGGTCAACGATAAGTCCCCCAGACGTAGTCATCCCAT5410.11781410197779607No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATTGCAGACAGGTT5400.11759633099447298No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5380.11716078902782678No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC5370.11694301804450367No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5270.1147653082112727No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5270.1147653082112727No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5230.1138942242779803No Hit
ACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC5220.1136764532946572No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5170.1125875983780417No Hit
CACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTA5160.11236982739471861No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5150.11215205641139551No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5140.11193428542807242No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGCCACCTTCATCAG5110.11128097247810312No Hit
GTTTACTACTGTGCGAGAGATGGGATGACTACGTCTGGGGGACTTATCGTTGACCTACCTCTTTACTACTGGGGC4990.10866772067822596No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC4980.10844994969490285No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTACTGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCC4940.10757886576161046No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4840.10540115592837947No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4840.10540115592837947No Hit
CCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATTGCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT4770.10387675904511778No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT4750.10344121707847159No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT4750.10344121707847159No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTC4740.1032234460951485No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4710.1025701331451792No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4690.102134591178533No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4670.10169904921188681No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4660.10148127822856372No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC4660.10148127822856372No Hit
GGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAGTAACTTAGCCTGGTACCAGCAGAAA4660.10148127822856372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4660.10148127822856372No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC4640.10104573626191751No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT4640.10104573626191751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC452.9962393E-853.7273341
ATACGTA200.007614306651.3006211
ACATGCG507.355993E-847.93801527
GGTATCA3150.047.7616041
GTATGCC451.8291794E-646.10778442
TCGTATG551.4617581E-743.92972640
ACGAGAC551.5699334E-743.53254322
TGTTAGA3800.041.57711870
GTATCAA8900.041.4925881
ATCTCGT602.9526564E-740.1673637
GTGCTAG451.05470564E-437.9921841
ATGCCGT557.184901E-637.78697244
AATGGTA1652.2992026E-937.46870870
CGAGACA655.806578E-736.8472723
ATCTCCG655.826423E-736.83121510
CTTATAC655.836373E-736.8231931
TATCAAC9900.036.610652
ATGCGCA754.2617103E-836.54811529
ATCAACG10450.035.010983
GCCGTCT601.28791435E-534.70313646
Produced by FastQC (version 0.11.5)