FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459198
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTACACTTTTGGCC21690.47234526282779976No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17880.3893745181816994No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17280.37630825918231353No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15480.3371094821841559No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11930.25980078310445603No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11830.257623073271225No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11410.24847669197165492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11000.2395480816554079No Hit
GTGTTAGCACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT10960.2386769977221155No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10770.23453934903897664No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10750.23410380707233044No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10420.2269173646226682No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10100.2199486931563291No Hit
GTATGGTAGCTCACCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGG9330.20318032744045053No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATTGCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9210.20056707564057333No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGA8770.19098515237435704No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGCAATGCCAGTGGCCCTGCT8620.18771858762451055No Hit
GTCTGGGACAGACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATT8580.18684750369121816No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8560.18641196172457197No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8450.1840164809080179No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTACTGGTGCTAACACTCTGA8340.1816210000914638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8100.17639449649170946No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGCAATGCCA8000.17421678665847848No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7980.1737812446918323No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGGTGAGCTACCATA7920.1724746187918937No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7910.1722568478085706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7900.1720390768252475No Hit
GCTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT7780.16942582502537032No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC7780.16942582502537032No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7730.16833697010875484No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7660.16681257322549314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7420.1615860696257388No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7360.1602794437258002No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7350.1600616727424771No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7020.15287523029281486No Hit
GTCAGAGTGTTAGCACCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGC7000.15243968832616867No Hit
GGCCAAAAGTGTACATGGGAGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT6990.15222191734284557No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC6840.1489553525929991No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGC6830.148737581609676No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6740.1467776427597681No Hit
GACTTCACTCTCACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA6690.14568878784315265No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT6580.14329330702659854No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAGTAACTTAGCCTGGTACC6510.14176891014333687No Hit
GCCTATATGGAGCTGAGCGGCCTGAGATCTGAGGACACGGCCGTTTACTA6460.14068005522672136No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6210.13523578064364392No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6090.13262252884376674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5860.12761379622733549No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5850.1273960252440124No Hit
GTAGTAAACGGCCGTGTCCTCAGATCTCAGGCCGCTCAGCTCCATATAGG5800.12630717032739688No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC5690.1239116895108428No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5690.1239116895108428No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTTATGGTGAGAGTGAAGTCTGTC5630.12260506361090422No Hit
GTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA5570.12129843771096566No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC5470.11912072787773466No Hit
CACCATAAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCCATGTA5370.11694301804450367No Hit
ATATGGAGCTGAGCGGCCTGAGATCTGAGGACACGGCCGTTTACTACTGT5100.11106320149478002No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5090.11084543051145693No Hit
CCATATAGGCTGTGCTCGTGGATTCGTCCGCGGTAATGGAGACTCTGCCC5050.10997434657816454No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTACTGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGAGGCTCTTTCC5010.10910326264487215No Hit
GTTTACTACTGTGCGAGAGATGGGATGACTACGTCTGGGGGACTTATCGTTGACCTACCTCTTTACTACTGGGGC5000.10888549166154905No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA4990.10866772067822596No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4890.10649001084499497No Hit
GTAGTAAAGAGGTAGGTCAACGATAAGTCCCCCAGACGTAGTCATCCCAT4830.10518338494505637No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGCCACCTTCATCAG4820.10496561396173329No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4810.1047478429784102No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4800.1045300719950871No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT4790.104312301011764No Hit
GTACATGGGAGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAAT4780.10409453002844088No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4710.1025701331451792No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4700.1023523621618561No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4680.10191682019520991No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4670.10169904921188681No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4660.10148127822856372No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC4650.10126350724524061No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATTGCAGACAGGTT4650.10126350724524061No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA4640.10104573626191751No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4630.10082796527859442No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT4620.10061019429527132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)