FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258813
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC69360.5509952629977606No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56760.4509009678165065No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGCTGTCTGTTGAGTAA51210.40681181398666844No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGCTGTCTGTTGAGTAACAGAAGTAGT48400.38448919736291254No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48120.382264879692218No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41460.3293578950964123No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35630.2830444235958796No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGG35560.282488344178206No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35370.2809789857588061No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35200.2796285071730273No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC33510.26620316123204957No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC33060.2626283649755762No Hit
CCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT29690.23585711301043125No Hit
GGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCC28960.23005799908326335No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28690.22791312132937935No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG28280.22465608474014803No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27420.21782425189444343No Hit
GTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG26990.2144083354715911No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG26700.21210457788408604No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26640.21162793838322294No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26450.21011857996382305No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26140.20765594254269695No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25520.20273066770044476No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGC25510.20265122778363429No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24770.19677267393965586No Hit
ACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTT24640.19573995502111913No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24440.1941511566849087No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC23680.18811372300730927No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23280.1849361263348885No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23060.18318844816505708No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22850.18152020991203618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22410.17802485357237333No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22250.17675381490340505No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21910.1740528577318474No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21870.1737350980646053No Hit
GGATTATTACTGCTGTCTGTTGAGTAACAGAAGTAGTCAGCTTCATCCTC21770.17294069889650013No Hit
GTAATAATCCCGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT21200.1684126236383005No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAGA21070.16737990471976377No Hit
GTCCCTGACCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT21060.16730046480295327No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20250.1608658315413012No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC20100.15967423278914342No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19600.15570223694861746No Hit
GGTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACTCAA19300.1533190394443019No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18910.15022088268869166No Hit
GCTCTGGAGATGCATTGCCAAGAAAATATACTTCTTGGTACCAGCAGAGG18830.1495853633542075No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC17810.14148249183953454No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17810.14148249183953454No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17470.1387815346679769No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17090.13576281782917718No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17030.13528617832831405No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17010.13512729849469302No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC16860.13393569974253522No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16770.13322074049124055No Hit
GTACCAGCAGAGGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACA16760.13314130057443005No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16620.13202914173908278No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16250.12908986481709356No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16240.12901042490028305No Hit
CCTCATAGATGACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCAT16230.12893098498347252No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGACCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG16210.1287721051498515No Hit
GACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATT15930.12654778747915696No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15790.1254356286438097No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15630.12416458997484138No Hit
GAAAATATACTTCTTGGTACCAGCAGAGGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGAC15500.12313187105630463No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15430.122575791638631No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15360.12201971222095735No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15140.12027203405112594No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15040.11947763488302077No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACT14880.11820659621405245No Hit
CTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGA14720.11693555754508414No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14720.11693555754508414No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14670.11653835796103154No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14630.11622059829378946No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14570.11574395879292636No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14510.11526731929206323No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAGAAAATATACTTCTTGGTACCAGCAGAGGTCAGGCCAGGCCC14480.11502899954163168No Hit
GGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCT14240.11312244153817923No Hit
GGATGAAGCTGACTACTTCTGTTACTCAACAGACAGCAGTAATAATCCCGTATTCGGCGGGGGGACCAAGCTGAC13840.10994484486575846No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13730.10907100578084275No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13620.10819716669592704No Hit
GCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGGTCAGGG13490.1071644477773903No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13240.1051784498571273No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13180.1047018103562642No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13130.10430461077221159No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACTCAACAGACAGCAGTAATAATC13110.10414573093859056No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13080.103907411188159No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13030.10351021160410641No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12910.10255693260238018No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12880.10231861285194863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1050.055.97088241
TCGTATG1100.053.32126640
CCGCTTA200.00765370851.2388931
ACCGTAC1150.050.35767427
CGTACTA1200.048.3171129
GACCGTA1250.046.30879626
GGTATCA9550.045.587721
TAGATCT1350.043.11857234
ACTAGAT1450.040.0695732
GTACTAG1450.040.0104830
ACGAGAC1450.039.87855522
ATCTCGT1500.038.94376837
GTATCAA26550.038.048121
CGGGTAT451.0891212E-437.791917
TCTCGTA1550.037.73597738
GCCGTCT1650.035.89171646
TATACAC1900.035.8028563
ATGCCGT1650.035.78543544
AGGGGGG2800.035.7185770
GTATGCC1750.035.63422442
Produced by FastQC (version 0.11.5)