FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258813
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC70240.5579859756770863No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50370.40013886097458484No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGCTGTCTGTTGAGTAA48100.38210599985859695No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGCTGTCTGTTGAGTAACAGAAGTAGT46170.36677409591416676No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41910.3329326913528856No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40700.32332046141881277No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38770.3079885574743826No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35340.28074066600837455No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGG34220.2718433953255964No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32430.2576236502165135No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30530.24253006602251484No Hit
TCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC30410.24157678702078864No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCC30150.2395113491837151No Hit
GTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG28970.23013743900007388No Hit
CCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCCTCCGGGATCCCT28130.22346448598799026No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27180.21591769389099094No Hit
GGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGACCC26960.2141700157211595No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26730.21234289763451758No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26620.21146905854960185No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG26140.20765594254269695No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25720.20431946603665518No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24640.19573995502111913No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23580.18731932383920408No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC23560.18716044400558304No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG23410.18596884525342525No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23130.18374452758273072No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23090.18342676791548862No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22310.17723045440426816No Hit
GGATTATTACTGCTGTCTGTTGAGTAACAGAAGTAGTCAGCTTCATCCTC22170.1761182955689209No Hit
GTAATAATCCCGTATTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT21890.17389397789822633No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAATACGGGATTATTACTGC21830.17341733839736323No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21640.17190797997796337No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21270.16896870305597417No Hit
ACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTT21200.1684126236383005No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21000.16682382530209014No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20870.1657911063835534No Hit
GTCCCTGACCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT20540.16316958912880627No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19750.15689383570077525No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19460.1545900781132702No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19360.15379567894516502No Hit
GGTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACTCAA19270.15308071969387033No Hit
GGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC19270.15308071969387033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18890.1500620028550706No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18870.14990312302144956No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAGA18840.14966480327101803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18640.14807600493480763No Hit
GCTCTGGAGATGCATTGCCAAGAAAATATACTTCTTGGTACCAGCAGAGG18260.1450572880960079No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17800.14140305192272404No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC17770.14116473217229247No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC17630.1400525733369452No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17510.13909929433521898No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17300.13743105608219808No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17130.13608057749641925No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16900.1342534594097773No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16840.13377681990891419No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGACCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTTG16300.12948706440114616No Hit
CCTCATAGATGACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCAT16040.12742162656407266No Hit
GTACCAGCAGAGGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACA16000.1271038668968306No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15890.1262300278119149No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15510.12321131097311515No Hit
GACCAACACAGGGGCCTGGCCTGACCTCTGCTGGTACCAAGAAGTATATT15490.1230524311394941No Hit
GAAAATATACTTCTTGGTACCAGCAGAGGTCAGGCCAGGCCCCTGTGTTGGTCATCTATGAGGACAGCAAACGAC15460.12281411138906255No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15330.1217813924705258No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15290.12146363280328373No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15250.12114587313604165No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15180.120589793718368No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15140.12027203405112594No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15100.11995427438388387No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15010.11923931513258919No Hit
GGCCTGACCTCTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCT14930.11860379579810504No Hit
GGATGAAGCTGACTACTTCTGTTACTCAACAGACAGCAGTAATAATCCCGTATTCGGCGGGGGGACCAAGCTGAC14730.11701499746189466No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14680.11661779787784206No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAAGAAAATATACTTCTTGGTACCAGCAGAGGTCAGGCCAGGCCC14680.11661779787784206No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14670.11653835796103154No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14140.11232804237007402No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13980.11105700370110572No Hit
CTGCTGGTACCAAGAAGTATATTTTCTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTTTGTCCTGGGGA13810.10970652511532689No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13740.10915044569765327No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACT13180.1047018103562642No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTTCTGTTACTCAACAGACAGCAGTAATAATC13020.10343077168729589No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12900.10247749268556966No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12890.10239805276875914No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG12820.10184197335108551No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12620.10025317501487511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)