FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514882
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT61680.4071604256965229No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60360.39844687573025495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50560.33375536840493186No Hit
GTATTACTGTCATCAGACTAGTAGTTTACCCTACACTTTTGGCCAGGGGA46070.3041160961711869No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44560.2941483231037137No Hit
GACTAGTAGTTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT41640.27487289439045415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38000.2508446202410485No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36320.2397546475567074No Hit
GTGTAAGCTAGTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT35190.23229532069164463No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34150.22543009950610013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33800.22311968853019576No Hit
CAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA32790.21645250257115736No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32010.211303586681999No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30920.20410830678561104No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30730.20285408368440577No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30700.2026560484578997No Hit
CTACTAGTCTGATGACAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC29910.19744112082657264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29160.19249024016392036No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAGCGTATTACTGTCATCAGACTAGTAGTTTA29000.1914340522892212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28600.18879358260247334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27510.18159830270608535No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG26570.17539319894222785No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26130.1724886822868052No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA26080.17215862357596168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24510.16179478005547626No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA23950.15809812249402924No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23090.15242111266752129No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22390.14780029071571252No Hit
GTACTAGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTC21570.14238732785787936No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21570.14238732785787936No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21340.14086905778799932No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20770.1371063884843836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20270.13380580137594875No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20260.13373978963378005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20250.13367377789161136No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20070.13248556653257482No Hit
GCGTATTACTGTCATCAGACTAGTAGTTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA20060.13241955479040612No Hit
GGCCAAAAGTGTAGGGTAAACTACTAGTCTGATGACAGTAATACGCTGCA19870.1311653316892009No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTA19770.1305052142675139No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG19340.12766670935425994No Hit
GTGTAGGGTAAACTACTAGTCTGATGACAGTAATACGCTGCAGCATCTTC19070.1258843923157051No Hit
GGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT18800.1241020752771503No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18770.1239040400506442No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18580.12264981694943895No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17650.11651072492775015No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17590.11611465447473797No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17550.11585060750606319No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17520.11565257227955708No Hit
GTATATTACTGTGCAAGAGATTACTGTGATAGTAGTGGTCATCATACCTT17490.115454537053051No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT17460.1152565018265449No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17390.11479441963136401No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17240.11380424349883356No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17180.1134081730458214No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAG17130.11307811433497791No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16940.11182389123377265No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCC16610.10964550374220566No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16440.10852330412533782No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGC16410.10832526889883172No Hit
CTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCTTCTCAGGGGTCCCC16270.10740110450846996No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16040.10588283443858992No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15880.10482664656389078No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15800.10429855262654121No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15750.10396849391569772No Hit
GTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA15670.10344039997834814No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15530.10251623558798638No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15430.10185611816629941No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15410.10172409468196203No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT15170.1001398128699133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGT250.006157557554.13576569
GGTATCA18650.039.7144171
GTATCAA47050.037.966661
GTGGTAT5250.036.387341
TATCAAC53950.032.986622
CGTATGC4700.032.33395841
ATGCCGT4850.032.30766744
ATCAACG55850.031.9844463
TCGTATG4450.031.73350140
TCAACGC57050.031.3116764
CGTTGTC6900.031.1895963
TGTCGTA553.4070387E-431.1244338
GCGTTGT7000.031.12054662
CAACGCA57550.031.0989025
CCCTAAG5900.030.79642928
AACGCAG58750.030.4657046
GTATAGC450.005326922529.7908061
GACAGGT3950.029.696531
CTTATAC6700.029.5129091
CGTGTTC7250.029.51027352
Produced by FastQC (version 0.11.5)