FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514882
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61530.40617024956399245No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56590.37356044893265616No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT53120.3506543744001183No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42560.2809459746699743No Hit
GTATTACTGTCATCAGACTAGTAGTTTACCCTACACTTTTGGCCAGGGGA41910.27665521142900895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41530.27414676522659853No Hit
GACTAGTAGTTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37260.2459597513205649No Hit
CAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA36800.24292321118080484No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35940.2372462013542969No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34590.2283346161615228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32700.21585839689163908No Hit
GTGTAAGCTAGTACCAATGCTCTGACTGGCCCGGCAGGTGATGGTGACTT32430.21407607985308427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30410.20074170793500748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28820.19024584093018465No Hit
CTACTAGTCTGATGACAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC28600.18879358260247334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28440.18773739472777418No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28310.18687924207958112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28280.18668120685307504No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAGCGTATTACTGTCATCAGACTAGTAGTTTA26750.1765814103012644No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26260.17334683493499825No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25050.1653594141325859No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23830.15730598158800488No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23820.15723996984583619No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG22960.15156296001932823No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA22740.1501107016916169No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.14905451381691776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22580.14905451381691776No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21550.14225530437354195No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21480.14179322217836107No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA21340.14086905778799932No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21210.14001090513980627No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21050.13895471726510714No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT19880.13123134343136958No Hit
GTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA19740.1303071790410078No Hit
GTGTAGGGTAAACTACTAGTCTGATGACAGTAATACGCTGCAGCATCTTC19320.12753468586992253No Hit
GCGTATTACTGTCATCAGACTAGTAGTTTACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18900.12476219269883727No Hit
GTACTAGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTC18450.12179166430124591No Hit
GGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT18430.1216596408169085No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18200.12014137074702848No Hit
GGCCAAAAGTGTAGGGTAAACTACTAGTCTGATGACAGTAATACGCTGCA17900.11816101848196757No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG17890.11809500673979888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17870.11796298325546148No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17850.1178309597711241No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17770.11730286583377453No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17740.11710483060726842No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGACTTT17560.11591661924823188No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17560.11591661924823188No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17510.11558656053738839No Hit
GTATATTACTGTGCAAGAGATTACTGTGATAGTAGTGGTCATCATACCTT17380.11472840788919532No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17230.11373823175666486No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17030.11241799691329095No Hit
GTCTCCAGACTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGCCGGGCCAGTCAGAGCATTGGTA16510.1089853863205187No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16400.10825925715666303No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16250.10726908102413257No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15810.1043645643687099No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGC15700.10363843520485425No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15550.10264825907232379No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAG15310.10106397726027506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1253.178793E-739.79662770
GTATCAA46350.035.473711
GTGGTAT7100.035.373481
GGTATCA20300.035.3014531
TATCAAC52800.030.997042
ATCAACG54300.030.0085723
TCAACGC54600.029.8990484
GTTAGTC902.6149064E-729.7356629
GCTTGAC1251.7644197E-1029.4684681
CAACGCA55300.029.4600855
AACGCAG56200.029.107356
CGCAGAG58500.027.8485898
AGAGTAC59000.027.83294911
ACGCAGA58700.027.8106987
GCAGAGT60100.027.2741839
CAGAGTA60200.027.17061610
TGGTATC9200.026.9208762
CCTAACG757.5787706E-526.7753562
GCGTTGT6500.026.67419262
GACAGGT3950.026.2808551
Produced by FastQC (version 0.11.5)