FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28290.39391293813937756No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26740.372330574968079No Hit
GTCTCTGGTGGCTCCGTCGCCAATAATCATTACTACTGGGCCTGGGTCCG19700.2743048738545683No Hit
TCTCTGGAGCATCCACTTTGCAGAGCGGGGTCCCTTCAAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCACT18630.2594060812137364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18610.2591275991083003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18520.2578744296338378No Hit
ACGTACTACTGTCAAGAGACTTACAGTCTTCGCAACGGTTTTGGCCAGGGGACCAACCTGGATCTCAAACGAA17760.24729210962726564No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17450.24297563699300592No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16910.23545662014623098No Hit
GTACTACTGTCAAGAGACTTACAGTCTTCGCAACGGTTTTGGCCAGGGGA16850.23462117382992265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15680.21832997066191018No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15670.2181907296091921No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15440.21498818539667686No Hit
GTAGTACGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTTCCAGAGCCACTGCCA14930.20788689170805605No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14800.20607675802272138No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14700.2046843474955408No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCGCCAATAATCATTACTACTGGG14010.19507671485799502No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13970.1945197506471228No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13840.1927096169617881No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13750.1914564474873256No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13600.18936783169655474No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13550.18867162643296448No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13510.18811466222209225No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGCTCAGGGA13100.18240577906065203No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13060.1818488148497798No Hit
CTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGATGACCCAGTC12960.18045640432259924No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12770.17781082432095618No Hit
GACCAGGGAAAGCCCCGAAGTTACTGATCTCTGGAGCATCCACTTTGCAG12630.17586144958290342No Hit
GTGCAGCCACAGTTCGTTTGAGATCCAGGTTGGTCCCCTGGCCAAAACCG12580.17516524431931316No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12540.17460828010844093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12500.17405131589756873No Hit
CTTCAAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCG12500.17405131589756873No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12430.17307662852854233No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12350.1719627001067979No Hit
GTATTATTGTGGCAGACGTCGTTATGATACTGGTGGTTCATTCATTGACTTCTGGGGCCAGGGAACCCTGGTCAC12340.17182345905407984No Hit
CAGTAGTACGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTTCCAGAGCCACTGCCA12220.17015256642146318No Hit
GTTTTGGCCAGGGGACCAACCTGGATCTCAAACGAACTGTGGCTGCACCA12220.17015256642146318No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12050.16778546852525625No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12010.16722850431438402No Hit
CTCCAGAGATCAGTAACTTCGGGGCTTTCCCTGGTCTCTGCTGATACCAATTTAAAAACTTACTAACACTCTCA11950.1663930579980757No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11290.15720314851868405No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11250.15664618430781185No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11170.1555322558860674No Hit
GTAGGAGACAGAGTTGCCCTCACTTGCCGGGCAAGTGAGAGTGTTAGTAAGTTTTTAAATTGGTATCAGCAGAGA11150.1552537737806313No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGATCCAGGTTGGTCCCCTGGCCAAAACCGTTGCGAAGA11130.1549752916751952No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10560.14703855167026605No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACAT10410.14494993587949523No Hit
GGCCAAAACCGTTGCGAAGACTGTAAGTCTCTTGACAGTAGTACGTTGCG10080.1403549811397994No Hit
CCCTTGACCCGTCCAAGAGCCAGTTTTCCCTGAGCCTGAGCTCTGTGACC9960.13868408850718275No Hit
GTTCAGTGGCAGTGGCTCTGGAACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACGTA9830.13687395482184803No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9730.1354815442946675No Hit
CTGTAAGTCTCTTGACAGTAGTACGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTT9700.13506382113651333No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9640.134228374820205No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9440.13144355376584388No Hit
GACTTACAGTCTTCGCAACGGTTTTGGCCAGGGGACCAACCTGGATCTCAAACGAACTGTGGCTGCACCAT9440.13144355376584388No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9430.13130431271312581No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9420.13116507166040778No Hit
GGTCAAGGGATATGGTGACGCGACTCTTGAGGGACGGAGTGGAGTATGCA9420.13116507166040778No Hit
CCTTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTTGCCCTCACTTGCCG9420.13116507166040778No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9400.13088658955497165No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9390.1307473485022536No Hit
ACCTTGAAGGGACCCCGCTCTGCAAAGTGGATGCTCCAGAGATCAGTAACTTCGGGGCTTTCCCTGGTCTCTG9360.13032962534409945No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9360.13032962534409945No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAATGAATGAACCAC9340.13005114323866335No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGGCTCAGGGAAA9190.12796252744789252No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9070.12629163481527586No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9050.12601315270983976No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9010.12545618849896753No Hit
GAGTTGCCCTCACTTGCCGGGCAAGTGAGAGTGTTAGTAAGTTTTTAAATTGGTATCAGCAGAGACCAGGGAAAG8980.12503846534081337No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACGTACTACTGTCAAGAGACTTACAGTCTT8970.12489922428809531No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8870.12350681376091475No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8840.12308909060276059No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8830.12294984955004253No Hit
ACCATATCCCTTGACCCGTCCAAGAGCCAGTTTTCCCTGAGCCTGAGCTC8790.12239288533917032No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTTGCCCTCACTT8670.12072199270655366No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8640.1203042695483995No Hit
GTCTGCATCTGTAGGAGACAGAGTTGCCCTCACTTGCCGGGCAAGTGAGA8640.1203042695483995No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTCGCAACGTACTACTGTCAAG8630.12016502849568143No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGATGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCTCC8550.11905110007393699No Hit
ATCCTCTATGGGGCCCCTGCATACTCCACTCCGTCCCTCAAGAGTCGCGT8530.11877261796850089No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG8530.11877261796850089No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8510.11849413586306479No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8130.11320297585977869No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8080.11250677059618842No Hit
CTTACAGTCTTCGCAACGGTTTTGGCCAGGGGACCAACCTGGATCTCAAA8010.11153208322716203No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7940.11055739585813565No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7920.11027891375269953No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7870.10958270848910925No Hit
GGGTTCCCTGGCCCCAGAAGTCAATGAATGAACCACCAGTATCATAACGA7730.10763333375105649No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7530.10484851269669539No Hit
GACTGTAAGTCTCTTGACAGTAGTACGTTGCGAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT7510.10457003059125929No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7490.10429154848582316No Hit
GTCCAAGAGCCAGTTTTCCCTGAGCCTGAGCTCTGTGACCGCCGCAGACA7490.10429154848582316No Hit
GCAGAGACCAGGGAAAGCCCCGAAGTTACTGATCTCTGGAGCATCCACTTTGCAGAGCGGGGTCCCTTCAAGGTT7480.10415230743310512No Hit
TCCCTGGTCTCTGCTGATACCAATTTAAAAACTTACTAACACTCTCACTTGCCCGGCAAGTGAGGGCAACT7440.10359534322223289No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACGAGTCCTGGGCCCGACTCCTGCAGCTGCAC7410.10317762006407873No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7410.10317762006407873No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7310.1017852095368982No Hit
GTGTATTATTGTGGCAGACGTCGTTATGATACTGGTGGTTCATTCATTGA7300.10164596848418013No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7210.10039279900971763No Hit
GCATACTCCACTCCGTCCCTCAAGAGTCGCGTCACCATATCCCTTGACCCGTCCAAGAGCCAGTTTTCCCTGAGC7200.10025355795699958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT150.002490953568.031541
ATATGGC200.006240830353.9499262
CATGTTA200.006424573753.55563459
GTGGTAT1150.053.2420771
TGCAACG200.00776580951.0485426
TATACAC1100.043.2927973
ATCTCGT903.6379788E-1241.93691337
AATGGTA3550.040.3902570
GATCTCG955.456968E-1239.67954336
CGTATGC1150.039.01493541
GTATCAA9550.038.1119081
TCGTATG1200.037.34709540
GACCGAG1200.036.86838526
AGACCGA1200.036.8632525
CTTATAC1300.036.632371
GCCGTCT1250.036.12598846
ACCGAGG1250.035.4109227
GTATGCC1400.034.55715642
TATCAAC10550.034.499412
GTTTTCT400.002780062434.02050819
Produced by FastQC (version 0.11.5)