FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAACGCTCAAACTGCTCATTTGAAGATAGA46730.4164219790247367No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40030.35671670918810633No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34590.3082395945744841No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31390.27972364480176515No Hit
GATTATTACTGCTGCTCATATGCGGGCAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCACCGTCCTAGGT30380.2707232981547507No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30070.26796081552051854No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30060.2678717031774788No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGCCCGCATATGAGCAGCAGTAATAAT29910.2665350180318826No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29630.2640398724267697No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT28370.2528117172037616No Hit
GTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG28190.25120769502904616No Hit
GTCTATTACTGTGTGAAAGATCGTGAGGGAGTGGCTGGTTCCCTTGACTA26470.23588037202620973No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25830.23017718207166593No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25080.2234937563436849No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23800.21208737643459732No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23540.2097704555155639No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23460.20905755677124593No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC21790.19417579548360822No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT21610.19257177330889277No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGCCCGCATATGAGCAG21350.19025485238985937No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21250.1893637289594619No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG21090.18793793147082594No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGC20980.18695769569738874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20340.18125450574284493No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20140.17947225888205No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20010.1783137984225333No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19850.17688800093389737No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19620.17483841704398317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19190.17100658629327406No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAA19070.1699372381767971No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCAC18940.1687787777172804No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCA18720.16681830617040597No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17920.15968931872722622No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTG17680.1575506224942723No Hit
GTGTAACTGCCCGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC16840.15006518567893357No Hit
CAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA16230.144629332753509No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16110.14355998463703207No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15620.13919347982808447No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15470.13785679468248827No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15260.13598543547865358No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15190.13536164907737536No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCAC15060.13420318861785865No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15030.1339358515887394No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14590.13001490849499053No Hit
GACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCT14480.12903467272155333No Hit
CCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATTTGTTTC14470.1289455603785136No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATCATGAACTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA14360.12796532460507637No Hit
CAGTAATAGACAGCCGTGTCCTCAACGCTCAAACTGCTCATTTGAAGATA13800.12297503339485055No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCGGGCAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCAC13640.1215492359062146No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13630.12146012356317486No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAGTTCATGATTTATGATGTCAGTAAG13420.11958876435934017No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTCGCTATCCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA13200.11762829281246574No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13200.11762829281246574No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13150.11718273109726703No Hit
GCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13120.11691539406814778No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13000.11584604595167082No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG12580.11210332754400146No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12390.11041019302624627No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12170.10844972147937182No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12140.10818238445025258No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT12030.10720214867681538No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC11960.10657836227553714No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11540.10283564386786778No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG11340.10105339700707285No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11250.10025138591971512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG950.047.2648740
TCTGGAG13800.044.09794670
GGTATCA8450.042.213021
GTATCAA23400.041.9198761
ACGAGAC1150.041.75418522
CGTATGC1100.040.85632741
ATCTCGT1150.038.97144337
GTACAAT655.707534E-736.9363941
TATCAAC26700.036.481852
CACGAGA1350.035.56679521
ATCAACG28250.034.3587843
TAGAACC501.9267116E-434.298084
CGATCGC400.002672123534.2980810
CGGGTAT501.927216E-434.2965517
TCGCACG400.002672707234.2965513
CAACGCA29100.033.4730385
GCTACAT1350.033.11040532
AACGCAG29700.032.9137656
AGGGGGG2255.2750693E-1132.69386370
TGCCGTC1400.032.2229745
Produced by FastQC (version 0.11.5)