FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCAACGCTCAAACTGCTCATTTGAAGATAGA40350.35956830416537827No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35790.3189330757392537No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33840.3015561688465031No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32230.28720908161710385No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGCCCGCATATGAGCAGCAGTAATAAT28200.25129680737208593No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27770.2474649766213768No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26730.2381972929452431No Hit
GATTATTACTGCTGCTCATATGCGGGCAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCACCGTCCTAGGT26560.23668238311356743No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26240.23383078813629557No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT25480.22705825006527477No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25470.22696913772223504No Hit
GTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG24850.22144417245377074No Hit
GTCTATTACTGTGTGAAAGATCGTGAGGGAGTGGCTGGTTCCCTTGACTA22870.20379992853190088No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22540.20085922121158925No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22350.19916608669383407No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21670.19310644736713126No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20980.18695769569738874No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGCCCGCATATGAGCAG20850.18579923523787203No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20780.18517544883659381No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC20230.18027426996940774No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT19690.17546220344526142No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19420.17305617018318822No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19200.1710956986363138No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19130.1704719122350356No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG18280.16289736307665711No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAA18140.16164979027410065No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18120.16147156558802114No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17990.16031310512850447No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCAC17660.15737239780819282No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCAAAGACATAAGTGTAACTGC17420.15523370157523889No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16770.14944139927765535No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCA16690.1487285005333374No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16590.1478373771029399No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16400.1461442425851847No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTG16180.1441837710383103No Hit
GTGTAACTGCCCGCATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC15650.1394608168572037No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15600.13901525514200497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15470.13785679468248827No Hit
CCCCTGAGGGCCGCTTACTGACATCATAAATCATGAACTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA14950.13322295284442143No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14560.1297475714658713No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14210.12662863945948016No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14040.12511372962780445No Hit
CAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA13780.12279680870877106No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCGGGCAGTTACACTTATGTCTTTGGAACTGGGACCAAGGTCAC13560.12083633716189664No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13330.11878675327198246No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTCGCTATCCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGA13170.11736095578334652No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13020.11602427063775031No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12890.1148658101782336No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAGTTCATGATTTATGATGTCAGTAAG12830.11433113611999512No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCAC12720.1133509003465579No Hit
GCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCTCCTGCA12620.11245977691616044No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12290.10951906959584878No Hit
CCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATTTGTTTC12130.10809327210721284No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12080.10764771039201411No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11870.10577635118817943No Hit
CAGTAATAGACAGCCGTGTCCTCAACGCTCAAACTGCTCATTTGAAGATA11770.10488522775778195No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG11690.10417232901346399No Hit
GACTCAGCCTCGCTCAGTGTCCGAGTCTCCTGGACAGTCAGTCACCATCT11620.10354854261218574No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11440.1019445204374703No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11330.10096428466403311No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11310.10078605997795362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA200.007573536651.37520224
GGTATCA8050.045.9873921
GTATCAA23400.043.359751
TCTGGAG11650.041.94436370
TATCAAC27050.037.3556022
ATCAACG28850.035.6122133
CAACGCA29050.035.238155
GGGCGAC501.6644817E-435.1625158
AACGCAG29700.034.4669466
CGCAGAG31150.032.7526368
AGAGTAC31750.031.91233611
GCAGAGT32300.031.4805249
CAGAGTA32150.031.41022310
GTACCTA553.3828267E-431.1614931
GTGAGCG4500.031.02551570
TCGGTTG450.00438436131.00016251
CAAACGA450.00441855430.95098349
CGTCAGC450.00445788930.8949747
CCTAGAC450.004792043530.4418493
TCGGAGC604.3604232E-429.8402266
Produced by FastQC (version 0.11.5)