FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258184
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56630.4500931501274853No Hit
GAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGT53340.42394435154158694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52490.41718858290997185No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA46310.3680701709765821No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC40420.32125666834103755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39830.31656737011438707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39170.31132171447101536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38250.30400958842267906No Hit
CTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG37550.2984460142554666No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34310.2726946138243691No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32690.25981891360882037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32290.25663972837041327No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG31190.24789696896479368No Hit
GTCCCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC31100.2471816522861521No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30870.24535362077406803No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30840.2451151818811875No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30430.24185651701182023No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA30080.239074729928214No Hit
CTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA29410.2337495946538821No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28960.23017301126067413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28670.22786810196282897No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGA28090.22325828336713865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27440.21809210735472712No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27350.21737679067608554No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26980.214436044330559No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCATAGTGTTGGCAGTAATAAGTTG26980.214436044330559No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26720.21236957392559436No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26480.21046206278255009No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26370.20958778684198814No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25360.2015603441150102No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25240.2006065885434881No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC24790.1970300051502801No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24700.1963146884716385No Hit
CACTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC23090.1835184678870499No Hit
GTGTATTACTGTGCGAGAGTTAAGGGGGGTAGTGTTAGCAACTGCCTTGA23060.18328002899416937No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAA22760.18089564006536404No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22660.18010084375576227No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC22600.17962396597000121No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT21740.17278871770742593No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20460.16261532494452322No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20380.1619794878968418No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20250.1609462526943595No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19980.1588003026584347No Hit
GTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGG19570.15554163778906743No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA19570.15554163778906743No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19170.15236245255066033No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19100.15180609513393908No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18800.14942170620513376No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT18740.1489448284193727No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTGATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTG18580.14767315432400985No Hit
GTCAGAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGG18290.1453682450261647No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17940.14258645794255848No Hit
CCCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17930.1425069783115983No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17660.1403610282756735No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17420.13845351713262924No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACT17300.13749976156110713No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGT16980.13495641337038145No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16850.13392317816789912No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16780.1333668207511779No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16540.13145930960813362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16540.13145930960813362No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA16430.13058503366757168No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATGGGACGTTCGGCCAAGGGAC16350.12994919661989027No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG16300.12955179846508938No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16250.1291544003102885No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16160.12843908363164688No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15950.12677001138148317No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15950.12677001138148317No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15680.12462406134555837No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15600.12398822429787694No Hit
GCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG15490.12311394835731498No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15450.12279602983347428No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15350.12200123352387249No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15310.1216833150000318No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15220.1209679983213902No Hit
ACTTATTACTGCCAACACTATGGGACGTTCGGCCAAGGGACCAAGGTGGA15150.12041164090466897No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14710.11691453714242114No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14600.11604026120185919No Hit
GCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAACTACTC14540.11556338341609812No Hit
CTGCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAG14500.11524546489225741No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14430.11468910747553618No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14350.11405327042785475No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14340.11397379079689457No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14330.1138943111659344No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14300.11365587227305386No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14290.11357639264209368No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG14280.11349691301113352No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14230.11309951485633263No Hit
GCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT13950.11087408518944764No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13830.10992032961792551No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13720.10904605367736356No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13660.1085691758916025No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13510.10737698142719984No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13470.10705906290335912No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG13350.106105307331837No Hit
CCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCA13230.10515155176031488No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13210.1049925924983945No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13190.10483363323647416No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG13170.1046746739745538No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13070.10387987766495203No Hit
TACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA13050.10372091840303167No Hit
GTATTACTGTGCGAGAGTTAAGGGGGGTAGTGTTAGCAACTGCCTTGACAACTGGGGCCAGGGAACCCTGGTCAC12970.10308508135535026No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12900.102528723938629No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACC12790.10165444799806705No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC12730.10117757021230599No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12660.10062121279558475No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12650.10054173316462456No Hit
CCTTGGTCCCTTGGCCGAACGTCCCATAGTGTTGGCAGTAATAAGTTGCA12620.10030329427174405No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12610.10022381464078386No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC12590.1000648553788635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1550.046.1049771
TCTGGAG13750.044.96624870
ACGCGTA851.8189894E-1244.1175527
CGTAGTA1100.040.33412630
CGTATGC1050.039.46530541
GACGCGT955.456968E-1239.4578926
ACGAGAC955.456968E-1239.42180622
GTATCAA15350.037.9094921
TCGTATG1100.037.61833240
AGACGCG1001.0913936E-1137.47753525
CGCGTAG1051.8189894E-1135.72842828
TGTTAGA16300.035.5359770
GACAGGT2250.034.7860871
ATCTCCG1201.8189894E-1234.0312210
TCTCCGA1201.8189894E-1234.0312211
TATCAAC17150.033.7335662
CCGTCTT1253.6379788E-1233.4217747
CTTATAC1155.4569682E-1132.5503041
ATCTCGT851.2656892E-732.30024337
AATCTCG851.2777491E-732.26532736
Produced by FastQC (version 0.11.5)