FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258184
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56710.45072898717516674No Hit
GAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGT48020.38166118787077247No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45550.36202971902360864No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41220.32761503881785176No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40150.31911071830511273No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA38770.30814252923260826No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36620.2910544085761701No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34260.2722972156695682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34180.2716613786218868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34060.27070762305036467No Hit
CTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG33190.2637928951568292No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31210.24805592822671405No Hit
GTCCCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC30610.24328715036910342No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29560.23494178911828476No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28340.22524527414114312No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27880.22158921111697494No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27430.21801262772376695No Hit
CTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA27420.21793314809280678No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG27410.21785366846184662No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCCATAGTGTTGGCAGTAATAAGTTG27350.21737679067608554No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27210.21626407584264307No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27190.2161051165807227No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27070.21515136100920054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26850.21340280912807663No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA26450.21022362388966953No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGA25770.2048190089843775No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25320.2012424255911695No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA25240.2006065885434881No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24710.1963941681025987No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24560.19520197363819602No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24350.19353290138803228No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24210.19242018655458978No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23800.18916152168522252No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT22940.18232627342264723No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22440.17835229187463836No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22020.17501414737431092No Hit
GTGTATTACTGTGCGAGAGTTAAGGGGGGTAGTGTTAGCAACTGCCTTGA21510.17096068619534185No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20930.16635086759965156No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20770.16507919350428873No Hit
CACTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC20460.16261532494452322No Hit
GTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGG20370.16190000826588163No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19860.15784654708691256No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAAAGTGGGGTCCCAT19810.15744914893211168No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGTCTAGTTTAGAA19060.15148817661009836No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18660.14830899137169126No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18610.14791159321689037No Hit
CTGCCAACACTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT18010.14314281535927972No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTGATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTG17820.14163270237103634No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17550.13948675233511157No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17330.13773820045398763No Hit
CCCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17310.1375792411920673No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17290.13742028193014694No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17110.13598964857286375No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16880.13416161706077967No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16860.1340026577988593No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16610.13201566702485487No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16610.13201566702485487No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16580.13177722813197434No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16410.13042607440565132No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA16360.13002867625085043No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16330.1297902373579699No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16300.12955179846508938No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG16260.12923387994124866No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16160.12843908363164688No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACT16030.12740584842916455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15880.12621365396476192No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15650.12438562245267783No Hit
GTCAGAGTATGAGTAGTTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGG15390.12231915204771321No Hit
CTGCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAG15130.12025268164274859No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15080.1198552834879477No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14770.1173914149281822No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATGGGACGTTCGGCCAAGGGAC14730.11707349640434149No Hit
ACTTATTACTGCCAACACTATGGGACGTTCGGCCAAGGGACCAAGGTGGA14590.11596078157089902No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14520.11540442415417776No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14410.1145301482136158No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG14390.11437118895169546No Hit
GTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATCAGGCGT14270.11341743338017334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14220.11302003522537245No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14200.11286107596345209No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG14160.11254315743961137No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14150.11246367780865119No Hit
GCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG14040.11158940186808924No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14010.11135096297520872No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14000.11127148334424852No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13910.11055616666560694No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13890.11039720740368658No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13820.10984084998696535No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13550.10769489995104055No Hit
GCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAACTACTC13520.10745646105816001No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13480.1071385425343193No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGCCAC13360.10618478696279718No Hit
GGTCTCTGCTGATACCAGGCCAACCAACTACTCATACTCTGACTGGCCCGGCAAGTGATGGTGACT13140.10443623508167327No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12960.10300560172439006No Hit
CCTTGGTCCCTTGGCCGAACGTCCCATAGTGTTGGCAGTAATAAGTTGCA12960.10300560172439006No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12880.10236976467670866No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12820.1018928868909476No Hit
TACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA12740.10125704984326617No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG12710.10101861095038564No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12690.10085965168846528No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12660.10062121279558475No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12640.10046225353366439No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12640.10046225353366439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)