FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402145
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGGACAGTCGGCCTCCATCTCCTGTAGGTCTAGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGA26730.6646856233448134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25410.6318616419450696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20950.5209563714580562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16350.4065697696104639No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15830.3936391102711708No Hit
GTGTGTAGCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCC15350.38170311703490034No Hit
GCTACACACTGGCCGTGGTCGTGCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15050.3742431212622313No Hit
CGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCCAG14230.3538524661502692No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14230.3538524661502692No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13340.33172114535801767No Hit
GTATCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGTGGCAGCGGGTC13280.3302291462034838No Hit
GGCCGTGGTCGTGCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12540.31182782329756675No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12350.30710315930820975No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12340.3068544927824541No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12320.3063571597309428No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11970.2976538313294956No Hit
ACCACGGCCAGTGTGTAGCTTGCATGCAGTAATAAACTCCAACATCCTCA11900.29591316564920617No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC11880.2954158325976949No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11850.29466983302042793No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11740.29193450123711595No Hit
GGCCAGGCCTATCTCCAAGGCGCCTCATTTATAAGGTATCTAACCGGGAC11640.2894478359795596No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11480.2854691715674694No Hit
CTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCC11400.28347983936142435No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11310.28124184062962365No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11200.2785065088463116No Hit
GTCCCAGACAGATTCAGTGGCAGCGGGTCAGGCACTGATTTCACACTGAG10820.2690571808675975No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10810.2688085143418419No Hit
GTTTATTACTGCATGCAAGCTACACACTGGCCGTGGTCGTGCGGCCAAGGGACCAAGGTGGAAATCAAACGAA10710.2663218490842855No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10610.26383518382672916No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGATACCTTATAAATGAGGCGCCTTGGAGATAGGCCTGGCCTCTGTTGAAA10030.24941252533290229No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC9600.23871986472540999No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9410.2339952007360529No Hit
GATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTACACACTGG9310.23150853547849654No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9180.2282758706436733No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA9000.22379987318007188No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC8700.2163398774074028No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCGCTGCCA8650.21509654477862464No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGCACGAC8640.214847878252869No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT8580.2133558790983352No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGTCGGCCTCCATCTCCTGTAGGTCTAGTCAAGGCCTCGTATA8360.2078852155317112No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8300.2063932163771774No Hit
TTTATAAGGTATCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGTGGCAGCGGGTCAGGCACTGATTTCACA8240.20490121722264357No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG8080.2009225528105534No Hit
GTTAGATACCTTATAAATGAGGCGCCTTGGAGATAGGCCTGGCCTCTGTT7910.1966952218727076No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7910.1966952218727076No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7740.1924678909348618No Hit
CCTTATAAATGAGGCGCCTTGGAGATAGGCCTGGCCTCTGTTGAAACCAA7670.19072722525457234No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7670.19072722525457234No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC7560.18799189347126039No Hit
GTATACGAGGCCTTGACTAGACCTACAGGAGATGGAGGCCGACTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA7540.1874945604197491No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT7450.18525656168794838No Hit
AACCGGGACTCTGGGGTCCCAGACAGATTCAGTGGCAGCGGGTCAGGCAC7410.18426189558492584No Hit
CCTGTAGGTCTAGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCCAGGC7280.18102923075010258No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7230.1797858981213244No Hit
GTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCCAGGCCTATCTCCAAGGCGCCTCATTTATAAGGTAT7140.17754789938952367No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6990.17381790150318913No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6990.17381790150318913No Hit
GGCCTGGCCTCTGTTGAAACCAAGTCAAGTAGGTGTTTCCATCACTGTAT6850.17033657014261025No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6820.16959057056534335No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6780.1685959044623208No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6760.16809857141080956No Hit
AGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCCAGGCCTATCTCCAAGG6580.1636225739472081No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6510.16188190826691864No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6490.1613845752154074No Hit
CTCCCTGCCCGTCACCCTTGGACAGTCGGCCTCCATCTCCTGTAGGTCTA6440.1601412425866292No Hit
GGCCTATCTCCAAGGCGCCTCATTTATAAGGTATCTAACCGGGACTCTGG6320.15715724427756156No Hit
CCACTCTCCCTGCCCGTCACCCTTGGACAGTCGGCCTCCATCTCCTGTAG6270.15591391164878338No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6240.15516791207151648No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6180.15367591291698268No Hit
AGCTACACACTGGCCGTGGTCGTGCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6090.15143791418518196No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6050.15044324808215942No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6050.15044324808215942No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6040.1501945815564038No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5950.14795658282460308No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5900.1467132501958249No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5850.14546991756704672No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5790.1439779184125129No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT5790.1439779184125129No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA5760.143231918835246No Hit
CTGTATACGAGGCCTTGACTAGACCTACAGGAGATGGAGGCCGACTGTCC5720.14223725273222346No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5680.14124258662920092No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5580.13875592137164455No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5550.13800992179437765No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC5550.13800992179437765No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5480.1362692561140882No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT5450.1355232565368213No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5380.13378259085653185No Hit
GAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTACACACTGGCCGTGGTCGTG5340.1327879247535093No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5300.13179325865048677No Hit
CTCCAAGGCGCCTCATTTATAAGGTATCTAACCGGGACTCTGGGGTCCCA5220.1298039264444417No Hit
TTATTACTGCATGCAAGCTACACACTGGCCGTGGTCGTGCGGCCAAGGGA5180.12880926034141915No Hit
GGTCAGGCACTGATTTCACACTGAGAATCAGCAGGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAG5180.12880926034141915No Hit
GTTTCAACAGAGGCCAGGCCTATCTCCAAGGCGCCTCATTTATAAGGTATCTAACCGGGACTCTGGGGTCCCAGA5100.12681992813537407No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5100.12681992813537407No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5060.1258252620323515No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5050.1255765955065959No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT4990.12408459635206207No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4980.12383592982630642No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4970.12358726330055078No Hit
GGGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTACACACTGGCCGTGGTCGTGCGGCCAAGGGA4890.12159793109450572No Hit
GATTCAGTGGCAGCGGGTCAGGCACTGATTTCACACTGAGAATCAGCAGG4850.12060326499148318No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC4850.12060326499148318No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG4840.12035459846572753No Hit
GGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGTCGG4830.1201059319399719No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC4770.1186139327854381No Hit
GGTTTCAACAGAGGCCAGGCCTATCTCCAAGGCGCCTCATTTATAAGGTA4760.11836526625968245No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC4640.11538126795061483No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT4590.11413793532183665No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC4490.1116512700642803No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC4480.11140260353852466No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4460.1109052704870134No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA4420.10991060438399086No Hit
CACTGGCCGTGGTCGTGCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT4390.10916460480672394No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT4370.10866727175521268No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA4360.10841860522945704No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4350.1081699387037014No Hit
GGTCTAGTCAAGGCCTCGTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAACAGAGGCCAGGCCTATCTC4290.1066779395491676No Hit
GTCCCGGTTAGATACCTTATAAATGAGGCGCCTTGGAGATAGGCCTGGCC4250.10568327344614503No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCTGCTGATTCTCAGTGTGAAATCAGTGCCTGACCCGCTGCCA4230.10518594039463378No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4220.10493727386887813No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4160.10344527471434432No Hit
GTCGGCCTCCATCTCCTGTAGGTCTAGTCAAGGCCTCGTATACAGTGATG4160.10344527471434432No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC4140.10294794166283305No Hit
GAATCTGTCTGGGACCCCAGAGTCCCGGTTAGATACCTTATAAATGAGGCGCCTTGGAGATAGGCCTGGCCT4070.1012072759825436No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT4030.10021260987952106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA100.009202385132.8657470
TGCGTGA252.3917273E-672.0422855
CGTATGG353.1346462E-759.53576741
TATGGCG308.518833E-658.15962243
CGCGTAT309.090607E-657.5257539
CCTAACG950.056.3817862
ACAGGAT301.0887357E-555.8014071
ATGCCGG200.00695200952.4933744
TCCGCTA200.007610290351.306136
CTAACGC1050.051.012093
GTCCTGT200.00828531650.2150236
CACGACT200.00828531650.2150234
ACGACTG200.00828531650.2150235
ACGGGCT200.00828938950.20878216
CTTGAAA2050.047.71704557
TATACAC3350.046.9672853
TTATACA3300.046.6644672
TCGTATG2250.046.15672340
GCGTATG306.2014617E-446.1567240
CTTATAC3350.045.9736981
Produced by FastQC (version 0.11.5)