FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953627
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31790.33335884994866966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28720.3011659695037997No Hit
GTATAACATTGCCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27070.2838636070497165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25680.26928767746718585No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25590.26834391224241766No Hit
ATGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGATGAGAGTGAAATCTGTC22490.23583644338929163No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA21640.22692310515536998No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20990.2201070229764887No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18960.19881987401782877No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18650.19556912713251617No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAA18610.19514967592150811No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18530.19431077349949194No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18330.19221351744445153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18010.18885790775638692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17830.1869703773068506No Hit
GCATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC17650.18508284685731424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17290.18130778595824154No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16790.17606464582064055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16790.17606464582064055No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTT15940.16715130758671892No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15890.16662699357295882No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15870.16641726796745476No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15420.16169844184361393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15420.16169844184361393No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG15110.15844769495830133No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14900.1562455761005089No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14880.15603585049500485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14790.15509208527023668No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13810.14481553060053878No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT13680.14345231416476253No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT13190.13831403682991358No Hit
ACTTATTACTGTCAAAAGTATAACATTGCCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13180.13820917402716157No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGATGAGAGTGAAATCTGTCCCAGATCCACTGCCA12940.1356924667611131No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12930.13558760395836106No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12760.13380493631157675No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12470.13076391503176818No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12320.1291909729904879No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT12160.12751316814645558No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11960.1254159120914152No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11830.12405269565563895No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11650.12216516520610259No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC11450.1200679091510622No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11070.11608312264648546No Hit
GATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAT11020.11555880863272537No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10990.1152442202244693No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10960.11492963181621327No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10870.11398586659144508No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATG10560.11073511970613248No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGGCGAGTCAGG10550.11063025690338046No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10540.11052539410062845No Hit
GATTTCACTCTCATCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCAAAAGTATAACATTGCC10510.11021080569237239No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10450.10958162887586026No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9940.10423362593550728No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9910.10391903752725122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16700.035.323511
TAACGGC701.038652E-634.21478736
GTGGTAT2400.032.304191
CGCGCTA450.00447872930.8650144
TATCAAC19550.030.1708832
ATCAACG19450.030.1495513
AACGCAG19750.029.6915846
CAACGCA19900.029.6340225
TCAACGC20000.029.48744
AGAGTAC20150.028.62442411
ACGCAGA20500.028.6128067
CGCAGAG20800.027.7226378
AATGGTA8000.027.71086570
CAGAGTA21000.027.62498510
TTAACGG756.5919725E-527.3281835
GTACGTA650.00827471727.21967369
TGTTAGA9900.027.14256970
TGTCTCG500.00862356726.99104716
CGAGCGT500.00867003526.9613236
GAGTTTA2550.026.4382131
Produced by FastQC (version 0.11.5)