FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798007
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAACAACTCACTGTATCTG80040.4451595572208562No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA71820.39944227135934396No Hit
GTATATGAGAGTACTACTAGAACTAATGTATGAAATCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGCT63840.35505979676386135No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60130.3344258392764878No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG54480.3030021573887087No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51820.2882079991902145No Hit
CTTCTATCCCACACGTGACAGTAATAGTCGGCCGCATCCCCGGCTTCGAC45910.25533827176423673No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGCCC45900.25528265462815214No Hit
GGATTACCTGTGGGGGGAACAACATTGGAAGTGAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCC45630.25378099195386894No Hit
GGATAGAAGTGGTGATCATCCAGGGCTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGC44940.24994340956403394No Hit
GCCCTGGATGATCACCACTTCTATCCCACACGTGACAGTAATAGTCGGCC43440.24160083915134925No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGCCCTGGATGATCA42940.238819982347121No Hit
ATCCAGGGCTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC42630.2370958511284995No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39170.2178523220432401No Hit
CCCCTGTGCTGGTCGTCTATGATAATGGCGACCGGCCCTCAGGGATCCCT38410.21362541970081317No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37670.2095097516305554No Hit
GTATATTACTGTGCGAGAGATTCGGTGATTCGGGGACAAGGAATCTACTA36930.2053940835602976No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC36310.20194582112305456No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35410.19694027887544374No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35200.1957723190176679No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34990.19460435915989205No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34690.1929358450773551No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATAATGGCGACCGGCCC34590.19237967371650946No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC34430.1914897995391564No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34160.18998813686487315No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32280.179532115280975No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATAATGGCGACCGGCCCTCAGG31990.17791921833452262No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT31450.1749158929859561No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31390.17458219016944873No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31340.17430410448902592No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30940.1720794190456433No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATGACTTCTACATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGC30520.16974349933009159No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCCGGGAACACGG30020.16696264252586338No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC29950.1665733225732714No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT29930.16646208830110226No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29850.16601715121242575No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29750.1654609798515801No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29410.16356999722470492No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29270.162791357319521No Hit
CTATTACTGTCACGTGTGGGATAGAAGTGGTGATCATCCAGGGCTGTTCG28140.15650662094196519No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27870.15500495826768193No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTAC27610.15355891272948327No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC27480.1528358899603839No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27110.150778055925255No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26930.14977694747573284No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC26690.1484421362097033No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAG26380.14671800499108179No Hit
GGGTGGCCGTGTTCCCGGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCCATTATCATAGA25290.14065573715786422No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24860.13826420030622794No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA24700.1373743261288749No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24590.1367625376319447No Hit
CTCCAGGGACAACGCCAACAACTCACTGTATCTGCAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGTATATTA24570.13665130335977557No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA24560.136595686223691No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGCGGCCGACTAT24500.13626198340718362No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTCACTT24260.13492717214115407No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24230.13476032073290037No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24050.1337592122833782No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATAAT23570.1310895897513191No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23320.12969916134920498No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23100.12847558435534454No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC23080.12836435008317543No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG22680.12613966463979284No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22620.12580596182328543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22610.1257503446872009No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22490.1250829390541861No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22460.12491608764593241No Hit
CAGTAATAGTCGGCCGCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT22400.12458238482942502No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA22310.12408183060466395No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22230.12363689351598743No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA22050.12263578506646526No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22000.12235769938604243No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21940.12202399656953504No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC21730.12085603671175918No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21070.11718530573017791No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20950.11651790009716313No Hit
GTTTGGTCCCTCCGCCGAACAGCCCTGGATGATCACCACTTCTATCCCACACGTGACAGTAATAGTCGGCCGCAT20750.11540555737547184No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG20470.11384827756510403No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT20420.1135701918846812No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG20290.11284716911558186No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20130.11195729493822884No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20090.11173482639389057No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT20040.11145674071346774No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC19980.11112303789696035No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAGCCCTGGATGATCAC19520.10856464963707038No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG19310.10739668977929452No Hit
GTCTGGGGGAGGCTTGGTCAAGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAATGA19280.10722983837104083No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19270.10717422123495625No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19230.106951752690618No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC19210.10684051841844888No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19200.1067849012823643No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19120.10633996419368778No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG19110.10628434705760323No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18520.1030029360286139No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC18480.10278046748427566No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG18440.10255799893993739No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG18440.10255799893993739No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT18420.10244676466776825No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG18360.10211306185126086No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG18280.10166812476258434No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18260.10155689049041522No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT18220.10133442194607696No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18150.10094510199348501No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18040.1003333134965548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC200.00750704551.4911911
GGTATCA14750.049.1398851
TGGTATC4000.038.6291352
GTGGTAT4100.037.7026981
GTATCAA34550.035.4947361
TGACGCG400.002662078534.3255513
TATCAAC38950.031.1192912
CGGACCC19400.030.53062465
ATCAACG39750.030.4870553
TCAACGC40100.030.304044
CAACGCA41200.029.577455
AACGCAG41800.029.1528956
CAAGGGA21000.029.01934270
AATCCAC21300.028.95748534
GACCCAG20850.028.95653367
GCGTTGT21800.028.95452162
GGACCCA20800.028.60141866
GAGTTGT22650.028.52981453
TCATTTG22250.028.52250536
GCGGACC20600.028.51605864
Produced by FastQC (version 0.11.5)