FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428489
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60810.42569456257626065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56890.39825297919689967No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGACTA53570.3750116381715225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43500.3045175706638273No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41780.29247687591574034No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT40080.2805761892461195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38810.2716856762635204No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGGCCCTGGT37810.264685272340214No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA36430.2550247149260512No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35920.25145450892516497No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAA35610.24928438370894002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34440.24109391111867157No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34390.24074389092250625No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33640.23549358798002645No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG31160.21813258625022663No Hit
CCATAGATGAGGAGACTGGGAGGCTGGCCAGGTTTTTGTTGGTACCAGGC30710.21498240448473876No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGGCCTCTCACTTTCGGCGGAGGGA30470.21330230754314522No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30440.21309229542544605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29270.2049018228351776No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29170.20420178244284695No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28740.20119160875582523No Hit
GTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTA28490.19944150777499864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28300.1981114310295704No Hit
GAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATCTATGGTGCATC28260.19783141487263817No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27710.19398119271481964No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27680.19377118059712045No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC27270.19090101498856485No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27250.19076100691009873No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA26300.18411062318295765No Hit
GACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGG26060.18243052624136413No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23510.16457949623693285No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT23070.16149931851067806No Hit
CTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATCTATGGTGCATCCACCAGGGCCT22720.15904917713752084No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21890.15323884188117654No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21300.14910860356642577No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20440.1430882561923823No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20360.1425282238785178No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20360.1425282238785178No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19950.13965805826996217No Hit
CTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCT19630.13741792901450414No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19520.13664788458294044No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGG19430.13601784822984286No Hit
GTGTTGACACAGTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGGGC19180.13426774724901627No Hit
CACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAG19020.13314768262128726No Hit
CACCATCAACAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGGCCTCTCACTTT18980.132867666464355No Hit
GGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGC18310.12817739583573973No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18310.12817739583573973No Hit
ACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTA17960.12572725446258248No Hit
GCGTGGCACCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17870.1250972181094849No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17570.12299709693249301No Hit
GTAATATATGGCAGTGTCTTCAGGGTTTAGACTACTGATCTGTAGATAGG17510.12257707269709463No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17470.12229705654016236No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGA17240.1206869636378019No Hit
CAGTAATATATGGCAGTGTCTTCAGGGTTTAGACTACTGATCTGTAGATA17140.11998692324547128No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17070.11949689497083982No Hit
GCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGG16830.11781679802924629No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16680.11676673744075033No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16530.11571667685225438No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16430.11501663645992373No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16340.11438660010682616No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16170.11319653143986408No Hit
TCTATGGTGCATCCACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16120.11284651124369877No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16070.11249649104753344No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16050.11235648296906732No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15950.11165644257673667No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15690.10983633755667702No Hit
ACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATC15570.10899628908588026No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15470.1082962486935496No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGACTACCGGAGAAATTGTGTTGACACAGTCTC15340.10738619618351979No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15330.10731619214428673No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15310.10717618406582059No Hit
GTGCCACGCTGCTGACAGTAATAAATTGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTC15300.10710618002658753No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15240.10668615579118915No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15230.10661615175195607No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC15080.10556609116346014No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15050.10535607904576094No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14990.10493605481036257No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14970.10479604673189642No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14940.10458603461419723No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAGCC14830.10381599018263354No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14710.10297594171183677No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14460.10122584073101017No Hit
GGCTAGGTAGTTGGCAATACTCTGACTGGCCCGACAGGAGAGGGTGGCCC14380.10066580841714566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTA150.002602172367.2895414
GTATCGT200.008129382550.4636236
CGCATAA304.9429026E-448.33881859
CCGCATA305.0411373E-448.14630558
CGTATGC3350.046.3930341
GGTATCA9700.046.128261
TCGTATG3400.044.5588840
CCGTCTT3550.044.3579647
CTTATAC3700.043.6442151
TATCGTA551.5477599E-743.6223338
AATATCG551.5961268E-743.45187436
CACGAGA3650.043.33705521
ATCTCGT2950.042.9090237
TATACAC3850.042.8176163
ATGCCGT3600.042.47445344
TTATACA4100.041.0273362
AATCTCG3100.040.74864636
ATATCGT603.109617E-739.9131237
ACGAGAC4000.039.55060222
CGTCTTC3950.039.08826448
Produced by FastQC (version 0.11.5)