FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428489
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61730.4321349341857025No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50070.35051022443995017No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGACTA49010.3430897962812454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44150.30906783321397646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42860.3000373121529112No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATT40470.283306346776209No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36940.2585949209269375No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36240.25369463818062304No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGGCCCTGGT35810.25068446449360127No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAA35370.24760428676734647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34960.24473412115879087No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34030.2382237455101159No Hit
CATCTATGGTGCATCCACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA33190.23234340621453858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32440.22709310327205878No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30850.21596246103400166No Hit
GTCTTTGTCTCCAGGGGAAAGGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTA30610.21428236409240814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29340.20539185110980906No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGGCCTCTCACTTTCGGCGGAGGGA29090.20364175012898245No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28990.2029417097366518No Hit
GAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATCTATGGTGCATC28110.1967813542841422No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28100.19671135024490913No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28050.19636133004874382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27930.19552128157794704No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27780.1944712209894511No Hit
CCATAGATGAGGAGACTGGGAGGCTGGCCAGGTTTTTGTTGGTACCAGGC26980.188870897850806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26250.18376060298679234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26070.18250053028059718No Hit
GACTTCACTCTCACCATCAACAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGG25550.17886032024047788No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA25480.17837029196584642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25360.17753024349504967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24770.1734000051802989No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24650.17255995670950214No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24510.17157990016023925No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22440.15708906403899503No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22430.15701905999976198No Hit
CTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATCTATGGTGCATCCACCAGGGCCT20820.1457484096832387No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20780.14546839352630647No Hit
CTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCT20580.1440683127416452No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20460.14322826427084842No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19990.13993807442689443No Hit
GTGTTGACACAGTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGGGC19850.13895801787763154No Hit
GGGCCACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTACCAACAAAAACCTGGCCAGC19530.1367178886221735No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18660.13062753720889697No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18640.13048752913043082No Hit
GTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGG18590.1301375089342655No Hit
CACCATCAACAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTGGCACCTGGCCTCTCACTTT18300.12810739179650665No Hit
GCGTGGCACCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18240.12768736756110827No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18180.1272673433257099No Hit
GTGCCACGCTGCTGACAGTAATAAATTGCAAAATCTTCAGGCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTC17970.12579725850181556No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17780.12446718175638735No Hit
ACCCTCTCCTGTCGGGCCAGTCAGAGTATTGCCAACTACCTAGCCTGGTA17370.12159701614783174No Hit
CACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAG17340.12138700403013253No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17240.1206869636378019No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17200.12040694748086964No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17170.12019693536317047No Hit
GCTCTAGGCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGAGG17070.11949689497083982No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16870.11809681418617854No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGA16770.11739677379384791No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGAGACTACCGGAGAAATTGTGTTGACACAGTCTC16390.11473662030299149No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16330.11431659606759309No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16070.11249649104753344No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16030.11221647489060117No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16020.11214647085136813No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15950.11165644257673667No Hit
GTAATATATGGCAGTGTCTTCAGGGTTTAGACTACTGATCTGTAGATAGG15930.11151643449827055No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15460.10822624465431656No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15290.10703617598735446No Hit
ACCTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGTCTCCTCATC15240.10668615579118915No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15190.10633613559502383No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAGCC15180.10626613155579076No Hit
CAGTAATATATGGCAGTGTCTTCAGGGTTTAGACTACTGATCTGTAGATA15120.1058461073203924No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15090.1056360952026932No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14700.1029059376726037No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14480.10136584880947631No Hit
TCTATGGTGCATCCACCAGGGCCTCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT14460.10122584073101017No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC14430.101015828613311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)