FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215546
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10560.4899186252586455No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4960.23011329368209107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4880.22640178894528315No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC3980.18464736065619403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3970.18418342256409306No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA3650.16933740361686136No Hit
CTATTACTGTCAGCAGTGTGGTGACTCACCCCCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAACGAA3410.1582028894064376No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3160.14660443710391285No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3150.14614049901181186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG3090.14335687045920592No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA3090.14335687045920592No Hit
GTCTATTACTGTCAGCAGTGTGGTGACTCACCCCCGTACACTTTTGGCCA3050.14150111809080196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3000.13918142763029703No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2940.13639779907769106No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT2890.1340781086171861No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2870.13315023243298416No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG2820.13083054197247918No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA2800.12990266578827722No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2770.12851085151197425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT2730.1266550991435703No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCCCAGTAGGTTCTTTGCCTGGTACC2590.12015996585415642No Hit
CCTCTATGCTACATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA2550.11830421348575247No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2480.11505664684104554No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC2460.11412877065684354No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2450.11366483256474255No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT2400.11134514210423763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2390.11088120401213661No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.10995332782793464No Hit
CTTTTGGCCAGGGGACCAGGCTGGACATCAAACGAACTGTGGCTGCACCA2350.10902545164373266No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT2280.10577788499902573No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT2260.10485000881482376No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG2250.10438607072272277No Hit
GGCCAAAAGTGTACGGGGGTGAGTCACCACACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTG2160.10021062789381384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1900.044.409555
TCGTATG1800.044.2065140
CGTATGC1900.043.8823141
GCCGTCT1950.043.70799346
TGCCGTC1950.043.51228345
TACACAT1900.043.398155
ACGAGAC1950.042.29519322
TATACAC1950.042.2853783
ACCTAAC552.074521E-741.997341
CCGTCTT2050.041.68004247
ACACATC2000.041.2282456
ATGCCGT2050.041.15427444
GACCGAG1950.040.67892526
CACGAGA2050.040.22267521
TTGAAAA2400.040.18937758
CTGATCT1600.040.08785634
CTGCTTG2100.040.0662654
ACCGAGG2000.039.7358827
ATCTCGT1650.039.30250537
AGACCGA2100.039.30147625
Produced by FastQC (version 0.11.5)