FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215546
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20530.9524649030833325No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT4830.2240820984847782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4110.1906785558535069No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4080.18928674157720393No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA3790.1758325369062752No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3530.16377014651164948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3320.1540274465775287No Hit
CTATTACTGTCAGCAGTGTGGTGACTCACCCCCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAACGAA3240.15031594184072078No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT3150.14614049901181186No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA3090.14335687045920592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2980.13825355144609502No Hit
GTCTATTACTGTCAGCAGTGTGGTGACTCACCCCCGTACACTTTTGGCCA2980.13825355144609502No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA2890.1340781086171861No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2800.12990266578827722No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2760.12804691341987323No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT2760.12804691341987323No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2650.12294359440676236No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGTT2620.1215517801304594No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT2600.12062390394625741No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCCCAGTAGGTTCTTTGCCTGGTACC2560.11876815157785346No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2540.11784027539365147No Hit
CCTCTATGCTACATCCAACAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA2530.11737633730155048No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2490.11552058493314653No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2440.11320089447264158No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT2440.11320089447264158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG2390.11088120401213661No Hit
CTTTTGGCCAGGGGACCAGGCTGGACATCAAACGAACTGTGGCTGCACCA2280.10577788499902573No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2240.10392213263062176No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT2210.1025303183543188No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT2170.10067456598591483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT258.716153E-6109.1392470
CCTAACG550.066.136352
CTAACGC550.066.09033
ACCTAAC600.060.6531681
GTACGTA408.971976E-657.62568769
GAACGGT200.00666196653.05285646
ACGCCAT700.051.9039966
TGGTACG402.2373513E-549.38887867
GGTACGT453.532083E-545.7156468
TACGGAT606.650935E-445.4746870
AACGCCA851.8189894E-1242.7444655
CGCCATG1001.4551915E-1136.3327947
GTCATAG559.820273E-636.0911451
ACGGGTA657.6275865E-735.57056816
ATCAGAA400.002451744434.8917942
GACCTCG400.00288630833.75023733
ATGCCCA400.002957461233.5830431
TTCGGAA502.397518E-433.0298111
AATGGTA1251.9038853E-532.7417770
TCCCGCA1052.6355652E-432.4819270
Produced by FastQC (version 0.11.5)