FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618946
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTTCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT35190.5685471753594012No Hit
GTATAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATAGTGAGAGTGAAGTCTGTC27630.44640404817221535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21710.35075757820553005No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19160.30955850752731257No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17090.2761145560355831No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT16610.2683594368490951No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15660.25301076345917095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15650.2528491984761191No Hit
CTCCTAGGCTACTCATTTCCTGGGCATCTGCCCGGGAATTCGGGGTCCCT15520.25074885369644523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14710.23766209006924674No Hit
GCCTAGGAGGCTGTCCTGGCTTCTTCTGATACCAAGCTAAGTAGTCGTTA13240.21391203756062727No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13000.21003447796738325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12340.19937118908596227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.19420110962830361No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11060.1786908712553276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10460.1689969722722176No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10390.16786601739085477No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9900.15994933322131494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9630.15558707867891544No Hit
GTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA9520.15380986386534526No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9390.15170951908567146No Hit
AGCCAGGACAGCCTCCTAGGCTACTCATTTCCTGGGCATCTGCCCGGGAA9170.14815508945853112No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9160.14799352447547928No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA9150.14783195949242744No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9110.1471856995602201No Hit
GTTTATTACTGTCAACAGTATTATACTTCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA9060.14637787464496096No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8810.14233875006866512No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8760.14153092515340596No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8350.13490676084828077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8080.1305445063058813No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8000.12925198644146663No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7920.12795946657705196No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7880.12731320664484463No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7840.12666694671263728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7580.12246625715328961No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7100.11471113796680163No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7040.11374174806849063No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6940.11212609823797229No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGCGGGC6920.11180296827186863No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT6900.11147983830576495No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6740.10889479857693564No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6700.1082485386447283No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6620.10695601878031363No Hit
GGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTGGTATTCTTTCAACA6620.10695601878031363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6500.10501723898369163No Hit
GTCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCAGA6450.10420941406843245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6420.10372471911927696No Hit
GTCCTGGCTTCTTCTGATACCAAGCTAAGTAGTCGTTATTCCGATTGTTG6420.10372471911927696No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG6380.10307845918706965No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6360.10275532922096597No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA6330.10227063427181048No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6320.10210906928875864No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6320.10210906928875864No Hit
GTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGCGGGCCACCATCAACT6310.1019475043057068No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG6300.10178593932265498No Hit
CTCCCTGTCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTGGTATTCTTTCAA6250.1009781144073958No Hit
GGCCAAAAGTGTGAGGGGAAGTATAATACTGTTGACAGTAATAAACTGCC6200.10017028949213663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)