FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618946
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACTTCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT35140.5677393504441421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29960.4840486892232925No Hit
GTATAATACTGTTGACAGTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATAGTGAGAGTGAAGTCTGTC28180.4552901222400662No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19190.31004320247646805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17890.28903975467972975No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16450.26577439712026574No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15500.25042572373034155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15160.24493251430657922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13390.21633551230640477No Hit
CTCCTAGGCTACTCATTTCCTGGGCATCTGCCCGGGAATTCGGGGTCCCT13310.2150429924419901No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12330.19920962410291043No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12290.19856336417070308No Hit
GCCTAGGAGGCTGTCCTGGCTTCTTCTGATACCAAGCTAAGTAGTCGTTA11700.18903103017064493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11260.18192217091636428No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11240.1815990409502606No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11190.18079121603500142No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10880.1757827015603946No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGAATTCCCGGGC10080.16285750291624795No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10080.16285750291624795No Hit
GTTTATTACTGTCAACAGTATTATACTTCCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA9970.16108028810267777No Hit
GTAATAAACTGCCACATCTTCAACCTGCAGGCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA9950.1607571581365741No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9690.15655646857722644No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9640.15574864366196728No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9150.14783195949242744No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9040.1460547446788573No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA8920.1441159648822353No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8460.13668397566185095No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8410.1358761507465918No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8200.1324832861025033No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8090.1307060712889331No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7970.12876729149231111No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7910.12779790159400012No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGTCTGTGTCTCTGGGCGAGCGGGC7620.12311251708549695No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7590.12262782213634145No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7310.11810400261089013No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6870.11099514335660945No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6850.1106720133905058No Hit
GTCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCAGA6600.10663288881420996No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6560.10598662888200262No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG6550.1058250638989508No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6530.10550193393284713No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6520.10534036894979529No Hit
AGCCAGGACAGCCTCCTAGGCTACTCATTTCCTGGGCATCTGCCCGGGAA6490.10485567400063979No Hit
GTCCTGGCTTCTTCTGATACCAAGCTAAGTAGTCGTTATTCCGATTGTTG6380.10307845918706965No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6320.10210906928875864No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGCCTGCAGGTTGAAGATG6220.10049341945824031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG252.7860788E-454.28888334
ATCTAGT252.842343E-454.0695832
TATAGAC200.008649480549.674623
CGCTAGT606.491973E-945.24073834
ACGCTAG606.6029315E-945.1547733
GCTAGTT751.0077201E-940.7841835
GTATCAA31550.038.0003551
CTGGTTT1754.107278E-935.3180570
CGGGATT953.3469405E-1034.90450317
CGGATGA1201.3833758E-534.3369970
TCTAGTC400.002842411633.86607733
CGGGACG809.0165486E-833.56657429
GGATAGG907.701601E-933.127126
CTAGTTT959.93532E-932.2729636
AACGCAG37750.031.854696
CAACGCA37700.031.7135075
TCAACGC37600.031.7072074
ATCAACG38050.031.332223
TAGTTTC1001.5994374E-830.73338137
TATCAAC39000.030.6539152
Produced by FastQC (version 0.11.5)