FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138251
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGAAGTCCTCAGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCAT66260.5821211665968227No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTC54480.47862905457583604No Hit
GTTTTATACTACGCCAACAATAAGAACTACTTAGCCTGGTACCAGAAGAA50580.44436596146192714No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41610.3655608472999365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32120.282187320722758No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31940.2806059471944237No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29030.25504040848635323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27490.24151087941060453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25350.22271010524040832No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25080.2203380449479069No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23460.20610568319289857No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23040.2024158116267853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21750.1910826346737231No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21530.189149844805759No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21300.18712920085288745No Hit
GTTTATTACTGTCAGCAGTATTATAGAAGTCCTCAGACGTTCGGCCAAGGGACCGAGGTGGAAATCAAACGAA21210.18633851408872032No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21120.1855478273245532No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20870.18335147520186673No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20020.1758838779847327No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19690.17298469318278656No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19500.17131546556954486No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTCGGTCCCTTGGCCGAACGTC19050.1673620317487092No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18740.16463855511657796No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18720.16446284694676305No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18380.16147580805990946No Hit
CTCCTAAGCCTCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT17680.15532602211638732No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17320.1521632750597188No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA16940.14882481983323537No Hit
GTAAATGAGAGGCTTAGGAGGCTGTCCTGGTTTCTTCTGGTACCAGGCTA16910.148561257578513No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16900.14847340349360555No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16630.14610134320110416No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16490.14487138601239974No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG16460.14460782375767736No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16430.14434426150295498No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16210.14241147163499088No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT16150.14188434712554612No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15770.1385458918990627No Hit
GATTTCACTCTCACCATCAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGAAGT15510.13626168569146876No Hit
GGTCTGGAGTGGGTCTCCGGAATTACTGGTAGTGGTGTTAGAACTTTTTA15190.13345035497443009No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14880.13072687834229885No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14830.13028760791776156No Hit
CTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG14720.1293212129837795No Hit
GCTTAGGAGGCTGTCCTGGTTTCTTCTGGTACCAGGCTAAGTAGTTCTTATTGTTGGCGTAGTATAAAACACT14620.12844267213470492No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGAGGCTTAGGAGGCTGTCCTGGTTTCTTCTGGTA14450.12694915269127813No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGCAGCCATGACATAAGCTGGGTCCGCCAGGCTCCACGGAAGGGT14170.12448923831386927No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14040.1233471352100723No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACTACGCCAA13880.12194146985155295No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13800.12123863717229327No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13700.12036009632321869No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13620.11965726364395901No Hit
CTTCTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAAT13320.11702164109673525No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13210.1160552461627532No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13180.11579168390803082No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13080.11491314305895624No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13030.11447387263441894No Hit
GTCTGAGAGTCGAGGACACGGCCATGTACTTCTGTGCGAAAGAAACTGTT12900.11333176953062198No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12890.11324391544571452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12800.11245322868154739No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12770.112189666426825No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12550.11025687655886092No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12510.10990546021923109No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGTAGCC12460.1094661897946938No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12380.10876335711543413No Hit
TCTTCAGCCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12330.10832408669089683No Hit
TCAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTAT12150.10674271316256256No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12120.10647915090784019No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12050.10586417231348798No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12010.10551275597385813No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11980.10524919371913576No Hit
GCCATGTACTTCTGTGCGAAAGAAACTGTTGACAGTGACTACGAGGACTA11920.10472206920969102No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11850.10410709061533881No Hit
GCCAAGGGACCGAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC11820.10384352836061643No Hit
CAATAAGAACTACTTAGCCTGGTACCAGAAGAAACCAGGACAGCCTCCTAAGCCTCTCATTTACTGGGCATCTAC11790.10357996610589404No Hit
CTCCAGACCCTTCCGTGGAGCCTGGCGGACCCAGCTTATGTCATGGCTGC11740.10314069568135675No Hit
CACCATCAGTAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGAAGTCCTCAGACGTT11670.10252571708700454No Hit
AACCAGGACAGCCTCCTAAGCCTCTCATTTACTGGGCATCTACCCGGGAA11420.10032936496431806No Hit
GGCTACTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAT11400.10015365679450314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGT200.006829549652.7363854
ACGAACG200.006920743352.55979552
TGGTATC2450.050.0160832
GTGGTAT2600.047.1554151
TTATACC306.7515153E-445.3749963
GGTATCA8750.044.3710021
GTTTGCG405.402819E-542.56524721
GCTTATA350.001436269538.9219321
GTATCAA20650.036.1182331
AATGGTA3650.035.18718370
GAGTTAG400.00256985434.57212443
TCGTGTG400.00274191934.1182128
GCACAAT552.4758704E-432.8641465
GACAGGT2300.032.5759661
CACTTTA2100.032.403595
TATCAAC22850.031.8787172
GAGTTTA4000.031.5024381
GCTTGAT652.5360412E-531.4369451
TATATGA652.5452024E-531.420362
CCGCACA450.004172730731.31516655
Produced by FastQC (version 0.11.5)